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Gene Expression Profiling pp 27–40Cite as

Microarray Analysis of mRNAs: Experimental Design and Data Analysis Fundamentals

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 784))

Abstract

Microarray technology has made it possible to quantify gene expression of thousands of genes in a single experiment. With the technological advancement, it is now possible to quantify expression of all known genes using a single microarray chip. With this volume of data and the possibility of improper quantification of expression beyond our control, the challenge lies in appropriate experimental design and the data analysis.

This chapter describes the different types of experimental design for experiments involving microarray analysis (with their specific advantages and disadvantages). It considers the optimum number of replicates for a particular type of experiment. Additionally, this chapter describes the fundamentals of data analysis and the data analysis pipeline to be followed in most common types of microarray experiment.

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Author information

Authors and Affiliations

  1. Conway Institute, University College Dublin, Dublin, Ireland

    Jai Prakash Mehta

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  1. Jai Prakash Mehta
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Correspondence to Jai Prakash Mehta .

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Editors and Affiliations

  1. School of Pharmacy, and Pharmaceutical Sciences, Trinity College Dublin, East End Development 4/5, Dublin, 2, Ireland

    Lorraine O'Driscoll

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Mehta, J.P. (2011). Microarray Analysis of mRNAs: Experimental Design and Data Analysis Fundamentals. In: O'Driscoll, L. (eds) Gene Expression Profiling. Methods in Molecular Biology, vol 784. Humana Press. https://doi.org/10.1007/978-1-61779-289-2_3

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  • DOI: https://doi.org/10.1007/978-1-61779-289-2_3

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-288-5

  • Online ISBN: 978-1-61779-289-2

  • eBook Packages: Springer Protocols

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