Abstract
In most hospitals around the world FFPE (formalin fixed, paraffin embedded) tissues have been used for diagnosis and have subsequently been archived since decades. This has lead to a sizeable pool of this kind of tissues. Till quite recently it was not possible to use this congeries of samples for protein analysis, but now several groups described successful protein extraction from FFPE tissues. In this chapter, we describe a protein extraction protocol established in our laboratory combined with the use of reverse phase protein microarray.
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Wolff, C., Schott, C., Malinowsky, K., Berg, D., Becker, KF. (2011). Producing Reverse Phase Protein Microarrays from Formalin-Fixed Tissues. In: Korf, U. (eds) Protein Microarrays. Methods in Molecular Biology, vol 785. Humana Press. https://doi.org/10.1007/978-1-61779-286-1_9
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DOI: https://doi.org/10.1007/978-1-61779-286-1_9
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Online ISBN: 978-1-61779-286-1
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