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Impact of Substrates for Probe Immobilization

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Protein Microarrays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 785))

Abstract

Protein chips are becoming a key technology in proteomic research and medical diagnostics. Surface chemistry for immobilization of proteins forms the basis for assay design and determines the properties of protein microarrays. Optimal substrates provide a homogeneous environment for probes, preventing loss of biological activity and unspecific adsorption. Numerous immobilization approaches, based on covalent binding, affinity, or adsorption, have been proposed thus far, and these represent the toolbox for choosing optimized strategies for each individual application.

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  1. Health & Environment Department, Biosensor Technologies, AIT Austrian Institute of Technology GmbH, Seibersdorf, Austria

    Ursula Sauer

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  1. Ursula Sauer
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Correspondence to Ursula Sauer .

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  1. (DKFZ), Division of Molecular Genome Analysis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, Heidelberg, 69120, Germany

    Ulrike Korf

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Sauer, U. (2011). Impact of Substrates for Probe Immobilization. In: Korf, U. (eds) Protein Microarrays. Methods in Molecular Biology, vol 785. Humana Press. https://doi.org/10.1007/978-1-61779-286-1_24

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  • DOI: https://doi.org/10.1007/978-1-61779-286-1_24

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