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Neural Differentiation of Human ES and iPS Cells in Three-Dimensional Collagen and Martigel™ Gels

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Human Embryonic and Induced Pluripotent Stem Cells

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Abstract

In this chapter, we describe an effective and reproducible protocol for neural differentiation of human pluripotent stem cells in three dimensional (3D) collagen and MartigelTM gels. We have used this protocol to generate embryoid bodies (EBs) from dissociated suspension cultures of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) which are immobilized in 3D collagen or MartigelTM gels. The gel-entrapped EBs differentiated into robust Pax6+ neuroectodermal cells of neural rosettes in N2 neural-inducing medium, then into nestin+ neural progenitors and further differentiated into TuJ1+ neurons, GFAP+ astrocytes, and O4+ oligodendrocytes. This 3D neural differentiation model, derived from human ES and iPS cells, has potential applications in the study of early human embryonic development, drug screening, and cell therapy.

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Author information

Authors and Affiliations

  1. BioDefense and Emerging Infections, American Type Culture Collection, Manassas, VA, USA

    Eric Derby

  2. Stem Cell Center, American Type Culture Collection, Manassas, VA, USA

    Dezhong Yin & Wu Ma

  3. Renji Stem Cell Research Center, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China

    Wei-Qiang Gao

  4. Ectycell, Romainville, France

    Wu Ma

Authors
  1. Eric Derby
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  2. Dezhong Yin
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  3. Wei-Qiang Gao
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  4. Wu Ma
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Corresponding author

Correspondence to Wu Ma .

Editor information

Editors and Affiliations

  1. , College of Engineering, University of Arkansas, University of Arkansas 1, Fayetteville, 72701, Arkansas, USA

    Kaiming Ye

  2. , College of Engineering, University of Arkansas, University of Arkansas 1, Fayetteville, 72701, Arkansas, USA

    Sha Jin

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© 2011 Humana Press

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Derby, E., Yin, D., Gao, WQ., Ma, W. (2011). Neural Differentiation of Human ES and iPS Cells in Three-Dimensional Collagen and Martigel™ Gels. In: Ye, K., Jin, S. (eds) Human Embryonic and Induced Pluripotent Stem Cells. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-61779-267-0_32

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  • DOI: https://doi.org/10.1007/978-1-61779-267-0_32

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-266-3

  • Online ISBN: 978-1-61779-267-0

  • eBook Packages: Springer Protocols

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