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Human Embryonic and Induced Pluripotent Stem Cells pp 359–367Cite as

Chondrogenic Differentiation of hESC in Micromass Culture

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Abstract

Human adult cartilage has limited capacity for self-renewal. Accordingly, repair of cartilage tissue damaged as a result of acute traumatic injury or via chronic wear or degenerative disease, such as arthritis, is a major clinical problem. Human embryonic stem cells (hESCs) could provide an unlimited source of chondrogenic progenitors for cartilage repair. In order to realize this potential, it is necessary to develop methodo­logies for the directed differentiation of hESCs into chondrocytes. In this chapter, we describe culture systems and conditions, which we have developed for direct, progressive, and substantially uniform differentiation of pluripotent hESCs into the chondrogenic lineage.

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Author information

Authors and Affiliations

  1. Center for Regenerative Medicine and Skeletal Development, Department of Reconstructive Sciences, Department of Orthopaedic Surgery, University of Connecticut Health Center, Farmington, CT, USA

    Deborah Ferrari, Guochun Gong, Robert A. Kosher & Caroline N. Dealy

Authors
  1. Deborah Ferrari
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  2. Guochun Gong
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  3. Robert A. Kosher
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  4. Caroline N. Dealy
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Corresponding author

Correspondence to Caroline N. Dealy .

Editor information

Editors and Affiliations

  1. , College of Engineering, University of Arkansas, University of Arkansas 1, Fayetteville, 72701, Arkansas, USA

    Kaiming Ye

  2. , College of Engineering, University of Arkansas, University of Arkansas 1, Fayetteville, 72701, Arkansas, USA

    Sha Jin

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© 2011 Humana Press

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Ferrari, D., Gong, G., Kosher, R.A., Dealy, C.N. (2011). Chondrogenic Differentiation of hESC in Micromass Culture. In: Ye, K., Jin, S. (eds) Human Embryonic and Induced Pluripotent Stem Cells. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-61779-267-0_26

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  • DOI: https://doi.org/10.1007/978-1-61779-267-0_26

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-266-3

  • Online ISBN: 978-1-61779-267-0

  • eBook Packages: Springer Protocols

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