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Human Embryonic and Induced Pluripotent Stem Cells pp 173–190Cite as

Human Mesenchymal Stem Cells and iPS Cells (Preparation Methods)

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Abstract

Mesenchymal stem cells (MSCs) are adult stem cells which show differentiation capabilities toward various cell lineages, and the MSCs can be cryopreserved for a long term without noticeable loss of these capabilities. We have used bone marrow–derived MSCs for treatments of patients with osteoarthritis, bone necrosis, and bone tumor. However, their proliferation/differentiation capabilities are limited and show possible disadvantages for clinical use. On the other hand, induced pluripotent stem cells (iPSCs) have nearly unlimited proliferation and differentiation capabilities into a wide variety of cells throughout the body, and thus the iPSCs are expected to be used in regenerative medicine and drug discovery research. We have reported that the MSCs can be utilized for the generation of iPSCs. In this chapter, we describe the methods of MSC preparation from bone marrow and iPSCs generation from MSCs. We also focus on the generation from commercially available MSCs from adipose tissue.

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Author information

Authors and Affiliations

  1. Tissue Engineering Research Group, Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Amagasaki, Hyogo, Japan

    Hiroe Ohnishi, Yasuaki Oda & Hajime Ohgushi

Authors
  1. Hiroe Ohnishi
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  2. Yasuaki Oda
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  3. Hajime Ohgushi
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Corresponding author

Correspondence to Hajime Ohgushi .

Editor information

Editors and Affiliations

  1. , College of Engineering, University of Arkansas, University of Arkansas 1, Fayetteville, 72701, Arkansas, USA

    Kaiming Ye

  2. , College of Engineering, University of Arkansas, University of Arkansas 1, Fayetteville, 72701, Arkansas, USA

    Sha Jin

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© 2011 Humana Press

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Ohnishi, H., Oda, Y., Ohgushi, H. (2011). Human Mesenchymal Stem Cells and iPS Cells (Preparation Methods). In: Ye, K., Jin, S. (eds) Human Embryonic and Induced Pluripotent Stem Cells. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-61779-267-0_14

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  • DOI: https://doi.org/10.1007/978-1-61779-267-0_14

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-266-3

  • Online ISBN: 978-1-61779-267-0

  • eBook Packages: Springer Protocols

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