Skip to main content
SpringerLink
Log in
Book cover

Plant Kinases pp 199–215Cite as

Fluorescence Fluctuation Analysis of Receptor Kinase Dimerization

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 779))

Abstract

Receptor kinases are essential for the cellular perception of signals. The classical model for activation of the receptor kinase involves dimerization, induced by the binding of the ligand. The mechanisms by which plant receptors transduce signals across the cell surface are largely unknown but plant receptors seem to dimerize as well. In this chapter, we describe two fluorescence fluctuation techniques, fluorescence cross-correlation spectroscopy and photon counting histogram analysis, to study the oligomerization state of receptor kinases in living plant cells in a quantitative manner.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Schmidt, E. D., Guzzo, F., Toonen, M. A., and de Vries, S. C. (1997) Development124, 2049–2062

    PubMed  CAS  Google Scholar 

  2. Hecht, V., Vielle-Calzada, J. P., Hartog, M. V., Schmidt, E. D., Boutilier, K., Grossniklaus, U., and de Vries, S. C. (2001) Plant Physiol127, 803–816.

    Article  CAS  Google Scholar 

  3. Shah, K., Gadella, T. W., Jr., van Erp, H., Hecht, V., and de Vries, S. C. (2001) J Mol Biol309, 641–655.

    Article  CAS  Google Scholar 

  4. Karlova, R., Boeren, S., Russinova, E., Aker, J., Vervoort, J., and de Vries, S. (2006) Plant Cell18, 626–638.

    Article  CAS  Google Scholar 

  5. Albrecht, C., Russinova, E., Hecht, V., Baaijens, E., and de Vries, S. (2005) Plant Cell17, 3337–3349.

    Article  CAS  Google Scholar 

  6. Xia, Z., and Liu, Y. (2001) Biophys. J.81, 2395–2402.

    Article  CAS  Google Scholar 

  7. Bastiaens, P. I. H., and Squire, A. (1999) Trends Cell Biol.9, 48–52.

    Article  CAS  Google Scholar 

  8. Chen, Y., Müller, J.D., So, P.T. C. and Gratton, E. (1999) Biophys. J.77, 553–567.

    Article  CAS  Google Scholar 

  9. Hess, S. T., Huang, S., Heikal, A. A., and Webb, W.W. (2002) Biochemistry41, 697–705.

    Article  CAS  Google Scholar 

  10. Chen, Y., We, L. N., and Müller, J. D. (2003) Proc. Natl. Acad. Sci. USA.100, 15492–15497.

    Article  CAS  Google Scholar 

  11. Meseth, U., Wohland, T., Rigler, R., and Vogel, H. (1999) Biophys. J.76, 1619–1631.

    Article  CAS  Google Scholar 

  12. Schwille, P., Meyer-Almes, F. J., and Rigler, R. (1997) Biophys. J.72, 1878–1886.

    Article  CAS  Google Scholar 

  13. Russinova J., Borst, J. W., Kwaaitaal, M., Yin, Y., Caño-Delgado, A., Chory, J., and de Vries, S. C. (2004) Plant Cell16, 3216–3229.

    Article  CAS  Google Scholar 

  14. Rodríguez-Concepción, M., Yalovsky, S., Zik, M., Fromm, H., and Gruissem, W. (1999) EMBO J.18, 1996–2007.

    Article  Google Scholar 

  15. Hink, M. A., Borst, J. W. and Visser, A. J. W. G. (2003) Methods Enzymol.361, 93–111.

    Article  CAS  Google Scholar 

  16. Skakun, V. V, Hink, M. A., Digris, A. V., Engel, R., Novikov, E. G., Apanasovich, V. V. and Visser, A. J. W. G. (2005) Eur. Biophys. J.34, 323–334.

    Article  Google Scholar 

  17. Müller, J. D., Chen, Y., and Gratton, E. (2000) Biophys. J.78, 474–486.

    Article  Google Scholar 

  18. Rigler, R., Földes-Papp, Z., Meyer-Almes, F.J., Sammet, C., Völcker, M., and Schnetz, A. (1998) J. Biotechnol.63, 97–109.

    Article  CAS  Google Scholar 

  19. Hink, M. A., Shah, K., Russinova, E., de Vries, S. C., and Visser, A. J. W. G. (2008). Biophys. J.94, 1052–1062.

    Article  CAS  Google Scholar 

  20. Liu, P., Sudhaharan, T., Koh, R. M. L., Hwang, L. C., Ahmed, S. Maruyama, I. N., and Wohland, T. (2007) Biophys. J.93, 684–698.

    Article  CAS  Google Scholar 

  21. Gadella, T. W. J. Ed. (2009) Lab. Techn. Biochem. Mol. Biol.33, Elsevier, Amsterdam, the Netherlands, pp 534.

    Google Scholar 

  22. Shaner, N. C., Patterson G. H., Davidson M. W. (2007) J Cell Sci.120, 4247–4260.

    Article  CAS  Google Scholar 

  23. Maeder, C. I., Hink, M. A., Kinkhabwala, A., Mayr, R., Bastiaens, P.I.H., and Knop, M. (2007) Nat. Cell Biol.9, 1319–1326.

    Article  CAS  Google Scholar 

  24. Müller, J. D. (2004) Biophys. J.86, 3981–3992.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to thank Khalid Shah, Eugenia Russinova, Frank van Breukelen, and Jeroen Pouwels for assistance during the experiments and Jan Willem Borst for valuable discussions. This work was supported by the Council of Earth and Life Sciences of the Netherlands Organization for Scientific Research (ALW-NWO).

Author information

Authors and Affiliations

  1. Department of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy (LCAM), University of Amsterdam, Amsterdam, The Netherlands

    Mark A. Hink

  2. Department of Biochemistry, Wageningen University, Wageningen, The Netherlands

    Sacco C. de Vries

  3. Laboratory of Biochemistry and Microscopy Centre, Wageningen (MSCWU), Wageningen University, Wageningen, The Netherlands

    Antonie J. W. G. Visser

Authors
  1. Mark A. Hink
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sacco C. de Vries
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Antonie J. W. G. Visser
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mark A. Hink .

Editor information

Editors and Affiliations

  1. Leibniz Institute of Plant Biochemistry, Weinberg 3, Halle (Saale), 06120, Sachsen-Anhalt, Germany

    Nico Dissmeyer

  2. rue du Général Zimmer 12, Strasbourg, F-67000, France

    Arp Schnittger

Rights and permissions

Reprints and permissions

Copyright information

© 2011 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Hink, M.A., de Vries, S.C., Visser, A.J.W.G. (2011). Fluorescence Fluctuation Analysis of Receptor Kinase Dimerization. In: Dissmeyer, N., Schnittger, A. (eds) Plant Kinases. Methods in Molecular Biology, vol 779. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-61779-264-9_11

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-264-9_11

  • Published:

  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-1-61779-263-2

  • Online ISBN: 978-1-61779-264-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation