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Microsecond Resolution of Single-Molecule Rotation Catalyzed by Molecular Motors

  • Tassilo Hornung
  • James Martin
  • David Spetzler
  • Robert Ishmukhametov
  • Wayne D. FraschEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 778)

Abstract

Single-molecule measurements of rotation catalyzed by the F1-ATPase or the FoF1 ATP synthase have provided new insights into the molecular mechanisms of the F1 and Fo molecular motors. We recently developed a method to record ATPase-driven rotation of F1 or FoF1 in a manner that solves several technical limitations of earlier approaches that were significantly hampered by time and angular resolution, and restricted the duration of data collection. With our approach it is possible to collect data for hours and obtain statistically significant quantities of data on each molecule examined with a time resolution of up to 5 μs at unprecedented signal-to-noise.

Key words

F1-ATPase FoF1 ATP synthase Nanodiscs Gold nanorods Plasmon resonance Molecular motors Dark field microscopy Single molecule 

Notes

Acknowledgments

This work was supported by grants from National Institutes of Health (GM50202), DARPA, and AFOSR (FA9550-05-1-0424) to W.D.F.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Tassilo Hornung
    • 1
  • James Martin
    • 1
  • David Spetzler
    • 1
  • Robert Ishmukhametov
    • 1
  • Wayne D. Frasch
    • 1
    Email author
  1. 1.Faculty of Biomedicine and Biotechnology, School of Life SciencesArizona State UniversityTempeUSA

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