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Single-Molecule Measurements Using Microneedles

  • Toshio Yanagida
  • Yoshiharu IshiiEmail author
  • Akihiko Ishijima
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 778)

Abstract

Myosin is both an enzyme and a molecular motor that hydrolyzes ATP and interacts with actin filaments for force generation. Manipulation techniques with microneedles and laser traps have recently been developed to capture and manipulate the actomyosin interaction for the purpose of revealing the mechanics of this system. Combined with single-molecule imaging techniques, the coupling between chemical processes (ATP hydrolysis) and mechanical processes (myosin force generation) has been directly determined. In this chapter, we describe these two manipulation techniques, especially microneedle method, in detail.

Key words

Microneedle Single-molecule manipulation Laser trap Myosin Mechanochemical ­coupling Single-molecule imaging 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Toshio Yanagida
    • 1
  • Yoshiharu Ishii
    • 2
    Email author
  • Akihiko Ishijima
    • 3
  1. 1.Department of Frontier BiosciencesOsaka UniversitySuitaJapan
  2. 2.CREST JST Department of Frontier BiosciencesOsaka UniversitySuitaJapan
  3. 3.Institute of Multidisciplinary Research for Advanced MaterialsTohoku UniversitySendaiJapan

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