Abstract
The androgen receptor (AR) plays a pivotal role in the progression of prostate cancer from the androgen-dependent to the castration-resistant state, making it a potential target for therapy. In this chapter, we describe the preparation and use of sublines of LNCaP and C4-2 human prostate cancer cells which have been engineered to stably express a doxycycline (DOX)-inducible AR shRNA in order to study the in vitro and in vivo effects of AR knockdown.
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Acknowledgments
The authors acknowledge the work of Dr Ladan Fazli, Mary Bowden, and Fariba Ghaidi in the development of these techniques in our lab. We also thank Drs Greg Hannon, Alice Mui, and Rob Kay for providing the plasmids. This work was supported by grants from the Prostate Cancer Foundation and the Canadian Institutes of Health Research.
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Cheng, H., Leblanc, E., Rennie, P.S. (2011). In Vitro and In Vivo Silencing of the Androgen Receptor. In: Saatcioglu, F. (eds) Androgen Action. Methods in Molecular Biology, vol 776. Humana Press. https://doi.org/10.1007/978-1-61779-243-4_7
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DOI: https://doi.org/10.1007/978-1-61779-243-4_7
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