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Studying Chloroplast Protein Interactions In Vitro: An Overview of the Available Methods

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Chloroplast Research in Arabidopsis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 775))

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Abstract

The analysis of protein–protein interactions is essential for the understanding of the molecular events in enzymatic pathways, signaling cascades, or transport processes in the chloroplast. A large variety of methods are available, which range from qualitative assays allowing for screening for new interaction partners, and semiquantitative assays allowing for a rough description of the interaction between two partners, to quantitative assays that permit detailed determination of kinetic and thermodynamic parameters. We summarize the available technologies, describe their range of applications and pitfalls, and give some examples from chloroplast research. The described techniques are generic and thereby important and useful to study the interaction network of proteins in Arabidopsis thaliana. In addition, we refer the reader to detailed protocols published elsewhere for each method.

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Authors and Affiliations

  1. Molecular Cell Biology of Plants, Goethe-University, Frankfurt, Germany

    Joanna Tripp

  2. Molecular Cell Biology of Plants, Centre of Membrane Proteomics, Cluster of Excellence Macromolecular Complexes, Goethe-University, Frankfurt, Germany

    Enrico Schleiff

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  1. Joanna Tripp
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  2. Enrico Schleiff
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Correspondence to Enrico Schleiff .

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  1. , Department of Biology, University of Leicester, University Road, Leicester, LE1 7RH, United Kingdom

    R. Paul Jarvis

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Tripp, J., Schleiff, E. (2011). Studying Chloroplast Protein Interactions In Vitro: An Overview of the Available Methods. In: Jarvis, R. (eds) Chloroplast Research in Arabidopsis. Methods in Molecular Biology, vol 775. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-237-3_5

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  • DOI: https://doi.org/10.1007/978-1-61779-237-3_5

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