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Nitric Oxide Methods in Seed Biology

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Seed Dormancy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 773))

Abstract

The ubiquitous signaling molecule nitric oxide (NO) plays an important role in seed biology. Experiments with this biologically important gas require special provisions because NO in aerobic environments is readily converted into other oxides of nitrogen. In this chapter, we describe methods for the application of NO as a gas, and through the use of NO-donor compounds. We included information on the removal or reduction of NO with NO scavengers. Methods for detecting NO using NO-reactive fluorescent probes, and an apparatus incorporating an oxidizer column are also described.

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Author information

Authors and Affiliations

  1. Department of Horticulture, University of Wisconsin-Madison, Madison, WI, USA

    Paul C. Bethke

  2. Department of Plant Biology, University of Minnesota, 250 Biological Science Center, St. Paul, MN, USA

    Igor G. L. Libourel

  3. Department of Plant Biology, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno, Czech Republic

    Jan Vitecek

  4. Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA

    Russell L. Jones

Authors
  1. Paul C. Bethke
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  2. Igor G. L. Libourel
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  3. Jan Vitecek
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  4. Russell L. Jones
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Corresponding author

Correspondence to Paul C. Bethke .

Editor information

Editors and Affiliations

  1. Dept. Biological Sciences, Simon Fraser Unviersity, University Drive 8888, Burnaby, V5A 1S6, British Columbia, Canada

    Allison R. Kermode

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Bethke, P.C., Libourel, I.G.L., Vitecek, J., Jones, R.L. (2011). Nitric Oxide Methods in Seed Biology. In: Kermode, A. (eds) Seed Dormancy. Methods in Molecular Biology, vol 773. Humana Press. https://doi.org/10.1007/978-1-61779-231-1_22

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  • DOI: https://doi.org/10.1007/978-1-61779-231-1_22

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-230-4

  • Online ISBN: 978-1-61779-231-1

  • eBook Packages: Springer Protocols

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