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Cerebral Perfusion MRI in Mice

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In vivo NMR Imaging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 771))

Abstract

Perfusion MRI is a tool to assess the spatial distribution of microvascular blood flow. Arterial spin labeling (ASL) is shown here to be advantageous for quantification of cerebral microvascular blood flow (CBF) in rodents. This technique is today ready for assessment of a variety of murine models of human pathology including those associated with diffuse microvascular dysfunction. This chapter provides an introduction to the principles of CBF measurements by MRI along with a short overview over applications in which these measurements were found useful. The basics of commonly employed specific arterial spin-labeling techniques are described and theory is outlined in order to give the reader the ability to set up adequate post-processing tools. Three typical MR protocols for pulsed ASL on two different MRI systems are described in detail along with all necessary sequence parameters and technical requirements. The importance of the different parameters entering theory is discussed. Particular steps for animal preparation and maintenance during the experiment are given, since CBF regulation is sensitive to a number of experimental physiological parameters and influenced mainly by anesthesia and body temperature.

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Authors and Affiliations

  1. Centre de Résonance Magnétique Biologique et Médicale (CRMBM), UMR 6612, CNRS, Université de la Méditerranée, 13385, Marseille Cedex 05, France

    Frank Kober, Guillaume Duhamel & Virginie Callot

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  1. Frank Kober
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  2. Guillaume Duhamel
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  3. Virginie Callot
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Correspondence to Frank Kober .

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Editors and Affiliations

  1. ERC Project BiosensorImaging, Leibniz-Institut für Molekulare Pharmako, Robert-Roessle-Str. 10, Berlin, 13125, Germany

    Leif Schröder

  2. Inst. Klinische Radiologie, AG Experimentelle, Universität Münster, Haus Rosenbach Waldeyerstr. 1, Münster, 48149, Germany

    Cornelius Faber

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Kober, F., Duhamel, G., Callot, V. (2011). Cerebral Perfusion MRI in Mice. In: Schröder, L., Faber, C. (eds) In vivo NMR Imaging. Methods in Molecular Biology, vol 771. Humana Press. https://doi.org/10.1007/978-1-61779-219-9_6

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  • DOI: https://doi.org/10.1007/978-1-61779-219-9_6

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-218-2

  • Online ISBN: 978-1-61779-219-9

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