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Cell Migration pp 137–147Cite as

Endothelial Cell Migration Under Flow

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 769))

Abstract

The endothelial cells lining blood vessels are continuously exposed to fluid shear stress generated by pulsatile flow of blood. In order to minimise forces acting on their surface, endothelial cells adapt to shear stress by alignment and migration within the direction of flow. Failure to adapt to shear stress results in endothelial damage contributing to generation of atherosclerotic plaques or abnormal vessel repair. This chapter describes methods of generating laminar flow in vitro and studying endothelial cell alignment and motility under flow.

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References

  1. Chien, S., Li, S., and Shyy, Y. J. (1998) Effects of mechanical forces on signal transduction and gene expression in endothelial cells. Hypertension 31, 162–9.

    PubMed  CAS  Google Scholar 

  2. Urbich, C., Dernbach, E., Reissner, A., Vasa, M., Zeiher, A. M., and Dimmeler, S. (2002) Shear stress-induced endothelial cell migration involves integrin signaling via the fibronectin receptor subunits alpha(5) and beta(1). Arterioscler Thromb Vasc Biol 22, 69–75.

    Article  PubMed  CAS  Google Scholar 

  3. Azuma, N., Akasaka, N., Kito, H., Ikeda, M., Gahtan, V., Sasajima, T., and Sumpio, B. E. (2001) Role of p38 MAP kinase in endothelial cell alignment induced by fluid shear stress. Am J Physiol Heart Circ Physiol 280, H189–97.

    PubMed  CAS  Google Scholar 

  4. Braddock, M., Schwachtgen, J. L., Houston, P., Dickson, M. C., Lee, M. J., and Campbell, C. J. (1998) Fluid Shear Stress Modulation of Gene Expression in Endothelial Cells. News Physiol Sci 13, 241–246.

    PubMed  CAS  Google Scholar 

  5. Albuquerque, M. L., Waters, C. M., Savla, U., Schnaper, H. W., and Flozak, A. S. (2000) Shear stress enhances human endothelial cell wound closure in vitro. Am J Physiol Heart Circ Physiol 279, H293–302.

    PubMed  CAS  Google Scholar 

  6. Morawietz, H., Talanow, R., Szibor, M., Rueckschloss, U., Schubert, A., Bartling, B., Darmer, D., and Holtz, J. (2000) Regulation of the endothelin system by shear stress in human endothelial cells. J Physiol 525 Pt 3, 761–70.

    Article  PubMed  CAS  Google Scholar 

  7. MacIsaac, A. I., Thomas, J. D., and Topol, E. J. (1993) Toward the quiescent coronary plaque. J Am Coll Cardiol 22, 1228–41.

    Article  PubMed  CAS  Google Scholar 

  8. Tzima, E. (2006) Role of small GTPases in endothelial cytoskeletal dynamics and the shear stress response. Circ Res 98, 176–85.

    Article  PubMed  CAS  Google Scholar 

  9. Wojciak-Stothard, B., and Ridley, A. J. (2003) Shear stress-induced endothelial cell polarization is mediated by Rho and Rac but not Cdc42 or PI 3-kinases. J Cell Biol 161, 429–39.

    Article  PubMed  CAS  Google Scholar 

  10. Tzima, E., del Pozo, M. A., Shattil, S. J., Chien, S., and Schwartz, M. A. (2001) Activation of integrins in endothelial cells by fluid shear stress mediates Rho-dependent cytoskeletal alignment. Embo J 20, 4639–47.

    Article  PubMed  CAS  Google Scholar 

  11. Seebach, J., Dieterich, P., Luo, F., Schillers, H., Vestweber, D., Oberleithner, H., Galla, H. J., and Schnittler, H. J. (2000) Endothelial barrier function under laminar fluid shear stress. Lab Invest 80, 1819–31.

    Article  PubMed  CAS  Google Scholar 

  12. Seebach, J., Donnert, G., Kronstein, R., Werth, S., Wojciak-Stothard, B., Falzarano, D., Mrowietz, C., Hell, S. W., and Schnittler, H. J. (2007) Regulation of endothelial barrier function during flow-induced conversion to an arterial phenotype. Cardiovasc Res 75, 596–607.

    Article  PubMed  CAS  Google Scholar 

  13. Schwachtgen, J. L., Houston, P., Campbell, C., Sukhatme, V., and Braddock, M. (1998) Fluid shear stress activation of egr-1 transcription in cultured human endothelial and epithelial cells is mediated via the extracellular signal-related kinase 1/2 mitogen-activated protein kinase pathway. J Clin Invest 101, 2540–9.

    Article  PubMed  CAS  Google Scholar 

  14. Schnittler, H. J., Franke, R. P., Akbay, U., Mrowietz, C., and Drenckhahn, D. (1993) Improved in vitro rheological system for ­studying the effect of fluid shear stress on ­cultured cells. Am J Physiol 265, C289–98.

    PubMed  CAS  Google Scholar 

  15. Buschmann, M. H., Dieterich, P., Adams, N. A., and Schnittler, H. J. (2005) Analysis of flow in a cone-and-plate apparatus with respect to spatial and temporal effects on endothelial cells. Biotechnol Bioeng 89, 493–502.

    Article  PubMed  CAS  Google Scholar 

  16. Dunn, G. A., and Brown, A. F. (1986) Alignment of fibroblasts on grooved surfaces described by a simple geometric transformation. J Cell Sci 83, 313–40.

    PubMed  CAS  Google Scholar 

  17. Allen, W. E., Zicha, D., Ridley, A. J., and Jones, G. E. (1998) A role for Cdc42 in macrophage chemotaxis. J Cell Biol 141, 1147–57.

    Article  PubMed  CAS  Google Scholar 

  18. Zicha, D., Dunn, G., and Jones, G. (1997) Analyzing chemotaxis using the Dunn direct-viewing chamber. Methods Mol Biol 75, 449–57.

    PubMed  CAS  Google Scholar 

  19. Wells, C. M., and Ridley, A. J. (2005) Analysis of cell migration using the Dunn chemotaxis chamber and time-lapse microscopy. Methods Mol Biol 294, 31–41.

    PubMed  Google Scholar 

  20. Woolf N. The Pathology of Atherosclerosis, Elsevier (1982), p. 28.

    Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Experimental Medicine and Toxicology, Imperial College London, London, UK

    Beata Wojciak-Stothard

Authors
  1. Beata Wojciak-Stothard
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Corresponding author

Correspondence to Beata Wojciak-Stothard .

Editor information

Editors and Affiliations

  1. , Division of Cancer Studies, King's College London, London, SE1 1UL, United Kingdom

    Claire M. Wells

  2. Randall Division of Cell and, Molecular Biophysics, King's College London, London, SE1 1UL, United Kingdom

    Maddy Parsons

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Wojciak-Stothard, B. (2011). Endothelial Cell Migration Under Flow. In: Wells, C., Parsons, M. (eds) Cell Migration. Methods in Molecular Biology, vol 769. Humana Press. https://doi.org/10.1007/978-1-61779-207-6_10

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  • DOI: https://doi.org/10.1007/978-1-61779-207-6_10

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-206-9

  • Online ISBN: 978-1-61779-207-6

  • eBook Packages: Springer Protocols

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