The Generation of Embryoid Bodies from Feeder-Based or Feeder-Free Human Pluripotent Stem Cell Cultures

Stover, Alexander E.; Schwartz, Philip H.

doi:10.1007/978-1-61779-201-4_28

Alexander E. Stover³ &
Philip H. Schwartz⁴

Part of the book series: Methods in Molecular Biology ((MIMB,volume 767))

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Abstract

Embryoid body (EB) formation is a traditional method of inducing differentiation of pluripotent stem cells (PSCs). It is a routine in vitro test of pluripotency as well as the first stage in many differentiation protocols targeted toward the production of a specific lineage or cellular population, as in neural differentiation (see Chapters 29 and 30). The induction of differentiation via EB formation is fairly straightforward. However, depending on the specific PSC culture conditions – substrate, feeders, medium, and eventual cell type of interest – various methods are applied in order to most routinely obtain healthy EB cultures.

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References

Ungrin M.D., Joshi C., Nica A., Bauwens C., and Zandstra P.W. (2008) Reproducible, Ultra-High-Throughput Formation of Multicellular Organization from Single Cell Suspension-Derived Human Embryonic Stem Cell Aggregates. PLoS One 3(2):e1565.
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Acknowledgments

This work has been funded by the National Institutes of Health (T15HL074286, R21MH087925, R01HD059967). The NCI Preclinical Repository supplied FGF-2. We also recognize Richard Pepple for proof-reading this chapter.

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Authors and Affiliations

Neuroscience Laboratories, Centers for Neuroscience and Translational Research, CHOC Research Institute, Children’s Hospital of Orange County Research Institute, Orange, CA, USA
Alexander E. Stover
Neuroscience Laboratories, Centers for Neuroscience and Translational Research, Children’s Hospital of Orange County Research Institute, Orange, CA, USA
Philip H. Schwartz

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Alexander E. Stover
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Philip H. Schwartz
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Correspondence to Philip H. Schwartz .

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Editors and Affiliations

Research Institute, CHOC Human Neural Stem Cell Resource, Children's Hospital of Orange County, S. Main Street 455, Orange, 92868-3874, California, USA
Philip H. Schwartz
Beckman Research Institute, Center for Applied Technology Developmen, City of Hope, East Duarte Road 1500, Duarte, 91010, California, USA
Robin L. Wesselschmidt

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Stover, A.E., Schwartz, P.H. (2011). The Generation of Embryoid Bodies from Feeder-Based or Feeder-Free Human Pluripotent Stem Cell Cultures. In: Schwartz, P., Wesselschmidt, R. (eds) Human Pluripotent Stem Cells. Methods in Molecular Biology, vol 767. Humana Press. https://doi.org/10.1007/978-1-61779-201-4_28

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DOI: https://doi.org/10.1007/978-1-61779-201-4_28
Published: 02 July 2011
Publisher Name: Humana Press
Print ISBN: 978-1-61779-200-7
Online ISBN: 978-1-61779-201-4
eBook Packages: Springer Protocols

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