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Stem Cell Banks: Preserving Cell Lines, Maintaining Genetic Integrity, and Advancing Research

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Human Pluripotent Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 767))

Abstract

The ability to cryopreserve and successfully recover cell lines has been critical to the conservation of all cell lines, especially the preservation of pristine early-stage cultures and the preparation of well-characterized cell banks. Indeed, the systematic storage and establishment of cryopreserved banks of cells for the stem cell research community is fundamental to the promotion of standardisation in stem cell research and their use in clinical applications. In spite of the significant potential for the use of stem cells in research and therapy, they are challenging to maintain and have been shown to be unstable after prolonged culture that often results in permanent alterations in their genetic make-up, which ultimately alters the phenotype of the culture. This chapter will review the principles of cell bank production, techniques for the scale-up of human pluripotent stem cells, quality control, and characterisation methods for banked cell lines.

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Acknowledgements

The authors would like to thank the Medical Research Council (MRC), the Biotechnology and Biological Sciences Research Council (BBSRC), and the International Stem Cell Forum for funding and supporting the activities of the UK Stem Cell Bank.

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Authors and Affiliations

  1. UK Stem Cell Bank, Cell Biology and Imaging, National Institute for Biological Standards and Control, South Mimms, Hertfordshire, UK

    Lyn Healy, Lesley Young & Glyn N. Stacey

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  1. Lyn Healy
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  2. Lesley Young
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  3. Glyn N. Stacey
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Correspondence to Glyn N. Stacey .

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Editors and Affiliations

  1. Research Institute, CHOC Human Neural Stem Cell Resource, Children's Hospital of Orange County, S. Main Street 455, Orange, 92868-3874, California, USA

    Philip H. Schwartz

  2. Beckman Research Institute, Center for Applied Technology Developmen, City of Hope, East Duarte Road 1500, Duarte, 91010, California, USA

    Robin L. Wesselschmidt

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Healy, L., Young, L., Stacey, G.N. (2011). Stem Cell Banks: Preserving Cell Lines, Maintaining Genetic Integrity, and Advancing Research. In: Schwartz, P., Wesselschmidt, R. (eds) Human Pluripotent Stem Cells. Methods in Molecular Biology, vol 767. Humana Press. https://doi.org/10.1007/978-1-61779-201-4_2

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  • DOI: https://doi.org/10.1007/978-1-61779-201-4_2

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-200-7

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