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Immunocytochemical Analysis of Human Pluripotent Stem Cells

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Book cover Human Pluripotent Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 767))

Abstract

This chapter will describe the most common immunocytochemical method utilized in the stem cell field – using fluorescently tagged secondary antibodies to detect a primary antibody that is bound to an epitope on a molecule of interest. Secondary antibodies recognize the heavy chain of the primary antibody’s isotype. Generally, these methods employ an incubation period of the sample with the primary antibody, a series of washes to remove unbound primary antibody, a secondary incubation period of the sample with the fluorescently conjugated secondary antibody, followed by washes and preparation for microscopy.

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References

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Acknowledgments

We gratefully acknowledge intellectual contributions from Lisa A. Flanagan, Richard Pepple, Boback Ziaeian, and Theo Palmer.

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Authors and Affiliations

  1. Neuroscience Laboratories, Centers for Neuroscience and Translational Research, CHOC Research Institute, Children’s Hospital of Orange County Research Institute, Orange, CA, USA

    Hubert E. Nethercott

  2. Neuroscience Laboratories, Centers for Neuroscience and Translational Research, Children’s Hospital of Orange County Research Institute, Orange, CA, USA

    David J. Brick & Philip H. Schwartz

Authors
  1. Hubert E. Nethercott
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  2. David J. Brick
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  3. Philip H. Schwartz
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Corresponding author

Correspondence to Philip H. Schwartz .

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Editors and Affiliations

  1. Research Institute, CHOC Human Neural Stem Cell Resource, Children's Hospital of Orange County, S. Main Street 455, Orange, 92868-3874, California, USA

    Philip H. Schwartz

  2. Beckman Research Institute, Center for Applied Technology Developmen, City of Hope, East Duarte Road 1500, Duarte, 91010, California, USA

    Robin L. Wesselschmidt

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© 2011 Springer Science+Business Media, LLC

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Nethercott, H.E., Brick, D.J., Schwartz, P.H. (2011). Immunocytochemical Analysis of Human Pluripotent Stem Cells. In: Schwartz, P., Wesselschmidt, R. (eds) Human Pluripotent Stem Cells. Methods in Molecular Biology, vol 767. Humana Press. https://doi.org/10.1007/978-1-61779-201-4_15

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  • DOI: https://doi.org/10.1007/978-1-61779-201-4_15

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-200-7

  • Online ISBN: 978-1-61779-201-4

  • eBook Packages: Springer Protocols

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