Abstract
This chapter describes some of the major issues to be considered when setting up a laboratory for the culture of human pluripotent stem cells (hPSCs). The process of establishing a hPSC laboratory can be divided into two equally important parts. One is completely administrative and includes developing protocols, seeking approval, and establishing reporting processes and documentation. The other part of establishing a hPSC laboratory involves the physical plant and includes design, equipment and personnel. Proper planning of laboratory operations and proper design of the physical layout of the stem cell laboratory so that meets the scope of planned operations is a major undertaking, but the time spent upfront will pay long-term returns in operational efficiency and effectiveness. A well-planned, organized, and properly equipped laboratory supports research activities by increasing efficiency and reducing lost time and wasted resources.
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Acknowledgments
We would like to express our gratitude to all the people with whom we have built our laboratories and to all those in whose great laboratories we have had the privilege and pleasure of working.
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Wesselschmidt, R.L., Schwartz, P.H. (2011). The Stem Cell Laboratory: Design, Equipment, and Oversight. In: Schwartz, P., Wesselschmidt, R. (eds) Human Pluripotent Stem Cells. Methods in Molecular Biology, vol 767. Humana Press. https://doi.org/10.1007/978-1-61779-201-4_1
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DOI: https://doi.org/10.1007/978-1-61779-201-4_1
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Online ISBN: 978-1-61779-201-4
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