Abstract
The normal blood–brain barrier (BBB) consists of tight interendothelial cell junctions and adjacent astrocyte end feet separated by a basal lamina surrounding the endothelium. The interactions between the different cell types of BBB are disrupted in distinct patterns in the microenvironment of glioma. Malignant gliomas infiltrate the surrounding normal brain parenchyma; a process associated with vascular permeability (VP) and breakdown of the BBB. Herein, we describe methods to quantitatively measure glioma-induced vascular permeability, utilizing an orthotopic xenograft model of glioma.
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References
Iadecola, C. (2004), Neurovascular regulation in the normal brain and in Alzheimer’s disease. Nat Rev Neurosci,5(5): p. 347–60.
Simard, M. and M. Nedergaard (2004), The neurobiology of glia in the context of water and ion homeostasis. Neuroscience, 129(4): p. 877–96.
Abbott, N.J., L. Ronnback, and E. Hansson (2006), Astrocyte-endothelial interactions at the blood-brain barrier. Nat Rev Neurosci, 7(1): p. 41–53.
Lee, J., et al. (2009), Glioma-induced remodeling of the neurovascular unit. Brain Res, 1288: p. 125–34.
Lund, C.V., et al. (2006), Reduced glioma infiltration in Src-deficient mice. J Neurooncol, 78(1): p. 19–29.
Neuwelt, E.A. and S.I. Rapoport (1984), Modification of the blood-brain barrier in the chemotherapy of malignant brain tumors. Fed Proc, 43(2): p. 214–9.
Zhang, X., et al. (2002), Experiment and observation on invasion of brain glioma in vivo. J Clin Neurosci, 9(6): p. 668–71.
Winkler, F., et al. (2009), Imaging glioma cell invasion in vivo reveals mechanisms of dissemination and peritumoral angiogenesis. Glia, 57(12): p.1306-15
Lampson, L.A., M.A. Lampson, and A.D. Dunne (1993), Exploiting the lacZ reporter gene for quantitative analysis of disseminated tumor growth within the brain: use of the lacZ gene product as a tumor antigen, for evaluation of antigenic modulation, and to facilitate image analysis of tumor growth in situ. Cancer Res, 53(1): p. 176–82.
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Lee, J., Baird, A., Eliceiri, B.P. (2011). In Vivo Measurement of Glioma-Induced Vascular Permeability. In: Turksen, K. (eds) Permeability Barrier. Methods in Molecular Biology, vol 763. Humana Press. https://doi.org/10.1007/978-1-61779-191-8_28
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DOI: https://doi.org/10.1007/978-1-61779-191-8_28
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Online ISBN: 978-1-61779-191-8
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