Abstract
Alterations in endothelial permeability are a hallmark of inflammation as well as the underlying cause of many clinical syndromes. Quantifying changes in endothelial barrier properties to water and macromolecules can be an important means of assessing the degree of cellular injury and, conversely, the effect of therapies to attenuate the inflammatory cascade. We use a combination of an isolated organ system and two cell culture models to investigate mechanisms of endothelial barrier regulation under variety of experimental conditions. Each assay has its own experimental strengths and limitations and must be used appropriately for the questions being asked. When used collectively, they can provide significant insight into the molecular regulation of lung endothelial permeability.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Uhlig, S. and von Bethmann, A. N. (1997) Determination of vascular compliance, interstitial compliance, and capillary filtration coefficient in rat isolated perfused lungs. J Pharmacol Toxicol Methods. 37, 119–127
Parker, J. C. and Ivey, C. L. (1997) Isoproterenol attenuates high vascular pressure-induced permeability increases in isolated rat lungs. J Appl Physiol. 83, 1962–1967
Uhlig, S. and Wollin, L. (1994) An improved setup for the isolated perfused rat lung. J Pharmacol Toxicol Methods. 31, 85–94
Bhattacharya, J. (2007) Interpreting the lung microvascular filtration coefficient. Am J Physiol Lung Cell Mol Physiol. 293, L9-L10
Parker, J. C. and Townsley, M. I. (2004) Evaluation of lung injury in rats and mice. Am J Physiol Lung Cell Mol Physiol. 286, L231–246
Hubert, C. G., McJames, S. W., Mecham, I. and Dull, R. O. (2006) Digital imaging system and virtual instrument platform for measuring hydraulic conductivity of vascular endothelial monolayers. Microvasc Res. 71, 135–140
Dull, R. O., Jo, H., Sill, H., Hollis, T. M. and Tarbell, J. M. (1991) The effect of varying albumin concentration and hydrostatic pressure on hydraulic conductivity and albumin permeability of cultured endothelial monolayers. Microvasc Res. 41, 390–407
Florian, J. A., Kosky, J. R., Ainslie, K., Pang, Z., Dull, R. O. and Tarbell, J. M. (2003) Heparan sulfate proteoglycan is a mechanosensor on endothelial cells. Circ Res. 93, e136–142
Pahakis, M. Y., Kosky, J. R., Dull, R. O. and Tarbell, J. M. (2007) The role of endothelial glycocalyx components in mechanotransduction of fluid shear stress. Biochem Biophys Res Commun. 355, 228–233
Dull, R. O., Mecham, I. and McJames, S. (2007) Heparan sulfates mediate pressure-induced increase in lung endothelial hydraulic conductivity via nitric oxide/reactive oxygen species. American Journal of Physiology-Lung Cellular, and Molecular Physiology. 292, L1452–L1458
Kim, M. H., Harris, N. R. and Tarbell, J. M. (2005) Regulation of capillary hydraulic conductivity in response to an acute change in shear. Am J Physiol Heart Circ Physiol. 289, H2126–2135
Kim, M. H., Harris, N. R. and Tarbell, J. M. (2005) Regulation of hydraulic conductivity in response to sustained changes in pressure. Am J Physiol Heart Circ Physiol. 289, H2551–2558
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Giantsos, K., Cluff, M., Dull, R. (2011). Mechano-Transduction and Barrier Regulation in Lung Microvascular Endothelial Cells. In: Turksen, K. (eds) Permeability Barrier. Methods in Molecular Biology, vol 763. Humana Press. https://doi.org/10.1007/978-1-61779-191-8_20
Download citation
DOI: https://doi.org/10.1007/978-1-61779-191-8_20
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-190-1
Online ISBN: 978-1-61779-191-8
eBook Packages: Springer Protocols