Abstract
Highly optimized methods for skin segmentation are provided using tape stripping in combination with infrared absorption measurements for stratum corneum (SC) and cryosectioning for deeper skin layers. Furthermore, an example is calculated for demonstration of the respective procedures.
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Abbreviations
- A T :
-
Area of tape stripping (e.g. 1.767 cm2)
- \( {c}_{{\text{EX}}_{n}}\) :
-
Drug concentration in the extract of n-th pool of tape strips
- c l :
-
Drug concentration in skin for l-th pool of cuts
- c n :
-
Drug concentration in skin for n-th pool of tape strips
- \( {d}_{{n}_{n}}\) :
-
Stratum corneum (SC) depth after n n pools of tape strips
- d n * :
-
Absolute SC thickness of the n-th pool
- d rel(n) * :
-
Relative SC thickness removed per pool n
- d tot :
-
Total SC thickness
- d x :
-
Middle of segment
- h :
-
First strip belonging to the n-th pool
- i :
-
Tape strip number
- IR-D:
-
Infrared densitometry
- j :
-
Last strip belonging to the n-th pool
- L k * :
-
Depth in the deeper skin layers after pool k of cuts
- L l * :
-
Absolute thickness of pool l of cuts
- L l :
-
Depth into the deeper skin layers after pool l of cuts
- L tot :
-
Total thickness of the stripped skin
- \( {m}_{{\text{EX}}_{n}}\) :
-
Extracted amount of drug of pool n
- N max :
-
Total number of pools of cuts
- n max :
-
Total number of tape strips
- Q:
-
Cumulative drug amount in SC without strip 1
- SC:
-
Stratum corneum
- V Ex :
-
Volume of the extracting agent (e.g. 2.0 ml)
- w k :
-
Weight of the pool of cuts/rest of the skin
- x i :
-
Absorption of a tape strip measured by IR-D and corrected for absorption of empty tape
- x i*:
-
Absorption measured by IR-D non-corrected value
- \( {X}_{{N}_{\mathrm{max}}}\) :
-
Absorption of total number of tapes n max
- x 0 :
-
Absorption of an empty tape measured by IR-D
- x pool(n) :
-
Sum of absorption of strips measured by IR-D in pool n containing strips h − j measured by IR-D
- \( {W}_{{N}_{\mathrm{max}}}\) :
-
Weight of total number of pools of cuts
References
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Melero, A., Hahn, T., Schaefer, U.F., Schneider, M. (2011). In Vitro Human Skin Segmentation and Drug Concentration–Skin Depth Profiles. In: Turksen, K. (eds) Permeability Barrier. Methods in Molecular Biology, vol 763. Humana Press. https://doi.org/10.1007/978-1-61779-191-8_2
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DOI: https://doi.org/10.1007/978-1-61779-191-8_2
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