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Therapeutic Oligonucleotides pp 107–121Cite as

Light-Directed Delivery of Nucleic Acids

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 764))

Abstract

A major barrier within the field of non-viral gene therapy toward therapeutic strategies, e.g., tumor therapy, has been lack of appropriate specific delivery strategies to the intended target tissues or cells. In this chapter, we describe a protocol for light-directed delivery of nucleic acids through the use of photochemical internalization (PCI) technology. PCI is based on a photosensitizing compound that localizes to endocytic membranes. Upon illumination, the photosensitizing compound induces damage to the endocytic membranes, resulting in release of endocytosed material, i.e., nucleic acids into cytosol. The main benefit of the strategy described is the possibility for site-specific delivery of nucleic acids to a place of interest.

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Authors and Affiliations

  1. Department of Tumor Biology, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, Oslo, N-0310, Norway

    Sigurd Bøe, Lina Prasmickaite, Birgit Engesæter & Eivind Hovig

Authors
  1. Sigurd Bøe
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  2. Lina Prasmickaite
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  3. Birgit Engesæter
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  4. Eivind Hovig
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Corresponding author

Correspondence to Eivind Hovig .

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Editors and Affiliations

  1. Dept. Chemistry, Worcester State College, Chandler St. 486, Worcester, 01602, Massachusetts, USA

    John Goodchild

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Bøe, S., Prasmickaite, L., Engesæter, B., Hovig, E. (2011). Light-Directed Delivery of Nucleic Acids. In: Goodchild, J. (eds) Therapeutic Oligonucleotides. Methods in Molecular Biology, vol 764. Humana Press. https://doi.org/10.1007/978-1-61779-188-8_7

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  • DOI: https://doi.org/10.1007/978-1-61779-188-8_7

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