Abstract
Tight junctions of hepatocytes play crucial roles in the barrier to keep bile in bile canaliculi away from the blood circulation, which we call the blood–biliary barrier. Tight junction proteins of hepatocytes are regulated by various cytokines and growth factors via distinct signal transduction pathways. To investigate changes in expression and function of tight junction proteins including claudins via signal transduction pathways in hepatocytes during EMT induced by TGF-β, we examined effects of TGF-β on expression and localization of the integral tight junction proteins, claudin-1, -2, and occludin, as well as the fence function by using primary cultures of adult rat hepatocytes.
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Acknowledgments
This work was supported by Grants-in-Aid from the National Project “Knowledge Cluster Initiative” (2nd stage, “Sapporo Biocluster Bio-S”), Program for developing the supporting system for upgrading the education and research, the Ministry of Education, Culture, Sports Science, and Technology, and the Ministry of Health, Labor and Welfare of Japan.
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Kojima, T., Sawada, N. (2011). Expression and Function of Claudins in Hepatocytes. In: Turksen, K. (eds) Claudins. Methods in Molecular Biology, vol 762. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-185-7_16
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DOI: https://doi.org/10.1007/978-1-61779-185-7_16
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