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Cell Cycle Synchronization for the Purpose of Somatic Cell Nuclear Transfer (SCNT)

  • Yoel ShufaroEmail author
  • Benjamin E. Reubinoff
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 761)

Abstract

Somatic cell nuclear transfer (SCNT) is a technically and biologically challenging procedure during which a differentiated committed nucleus undergoes rapid reprogramming into the totipotent state in a few hours. SCNT can be utilized to generate patient- and disease-specific embryonic stem cell (ESC) lines, which carry great promise in improving our understanding of major disease conditions and hope for better therapies. In this section, we will describe how mouse SCNT is performed and survey the importance of donor cell cycle synchronization and the methods to perform it.

Key words

Somatic cell nuclear transfer reprogramming cell cycle synchronization 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Obstetrics and Gynecology and the Hadassah Human Embryonic Stem Cell Research Center, the Goldyne Savad Institute of Gene TherapyHadassah University HospitalJerusalemIsrael

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