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Cell Cycle Synchronization pp 227–238Cite as

Synchronization of Medicago sativa Cell Suspension Culture

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 761))

Abstract

Deepening our knowledge on the regulation of the plant cell division cycle depends on techniques that allow for the enrichment of cell populations in defined cell cycle phases. Synchronization of cell division can be achieved using different plant tissues; however, well-established cell suspension cultures provide the largest amount of biological sample for further analysis. Here we describe the methodology of the establishment, propagation, and analysis of a Medicago sativa suspension culture that can be used for efficient synchronization of the cell division and also the application and removal of hydroxyurea blocking agent. A novel method is used for the estimation of cell portion that enters S phase during the assay. The protocol can be used in the case of other species as well.

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Acknowledgment

The authors are grateful to Katalin Török for excellent technical assistance. This work was funded by OTKA grant no. NK 69227. Edit Ábrahám was supported by the János Bolyai Fellowship of the Hungarian Academy of Sciences.

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Authors and Affiliations

  1. Cellular Imaging Laboratory, Biological Research Centre, Hungarian Academy of Sciences, H-6726, Szeged, Temesvárt krt. 62, Hungary

    Ferhan Ayaydin & Edit Kotogány

  2. Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, H-6726, Szeged, Temesvárt krt. 62, Hungary

    Gábor V. Horváth

  3. Cellular Imaging Laboratory, Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, H-6726, Szeged, Temesvárt krt. 62, Hungary

    Edit Ábrahám

Authors
  1. Ferhan Ayaydin
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  2. Edit Kotogány
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  3. Edit Ábrahám
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  4. Gábor V. Horváth
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Corresponding author

Correspondence to Gábor V. Horváth .

Editor information

Editors and Affiliations

  1. , Dept. Microbial Biotech. & Cell Biology, University of Debrecen, Egyetem Square 1, Debrecen, 4010, Hungary

    Gaspar Banfalvi

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Ayaydin, F., Kotogány, E., Ábrahám, E., Horváth, G.V. (2011). Synchronization of Medicago sativa Cell Suspension Culture. In: Banfalvi, G. (eds) Cell Cycle Synchronization. Methods in Molecular Biology, vol 761. Humana Press. https://doi.org/10.1007/978-1-61779-182-6_15

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  • DOI: https://doi.org/10.1007/978-1-61779-182-6_15

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-181-9

  • Online ISBN: 978-1-61779-182-6

  • eBook Packages: Springer Protocols

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