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Neural Stem Cells for Developmental Neurotoxicity Studies

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In Vitro Neurotoxicology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 758))

Abstract

The developing nervous system is particularly susceptible to toxicants, and exposure during development may result in long-lasting neurological impairments. The damage can range from subtle to severe, and it may impose substantial burdens on affected individuals, their families, and society. Given the little information available on developmental neurotoxicity (DNT) and the growing number of chemicals that need to be tested, new testing strategies and approaches are necessary to identify developmental neurotoxic agents with speed, reliability, and respect for animal welfare. So far, there are no validated alternative methods for DNT testing. Recently, neural stem/progenitor cells have been proposed as relevant models for alternative DNT testing. In this chapter, we provide detailed protocols for culturing neural stem cells (NSCs), in vitro experimental models, including primary cultures of rat and human embryonic NSCs, rat and mouse adult NSCs, as well as the mouse NSC line C17.2 that we have implemented and successfully used for neurotoxicity studies.

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Author information

Authors and Affiliations

  1. Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

    Roshan Tofighi & Michaela Moors

Authors
  1. Roshan Tofighi
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  2. Michaela Moors
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  3. Raj Bose
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  4. Wan Norhamidah Wan Ibrahim
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  5. Sandra Ceccatelli
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Corresponding author

Correspondence to Roshan Tofighi .

Editor information

Editors and Affiliations

  1. , Department of Environmental and, University of Washington, Roosevelt Way NE 4225, Seattle, 98105, Washington, USA

    Lucio G. Costa

  2. , Department of Environmental and, University of Washington, Roosevelt Way NE 4225, Seattle, 98105, Washington, USA

    Gennaro Giordano

  3. Jesse Brown VA Medical Center, Department of Psychiatry, University of Illinois at Chicago, South Damen Avenue 820, Seattle, 60612, Illinois, USA

    Marina Guizzetti

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Tofighi, R., Moors, M., Bose, R., Ibrahim, W.N.W., Ceccatelli, S. (2011). Neural Stem Cells for Developmental Neurotoxicity Studies. In: Costa, L., Giordano, G., Guizzetti, M. (eds) In Vitro Neurotoxicology. Methods in Molecular Biology, vol 758. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-170-3_5

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  • DOI: https://doi.org/10.1007/978-1-61779-170-3_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-169-7

  • Online ISBN: 978-1-61779-170-3

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