Abstract
Calcium is an important intracellular ion involved in numerous cell processes. There are multiple factors that contribute to the release of Ca2+. Some factors induce release as part of intracellular signaling cascades, while others result in unwanted changes to both basal and inducible Ca2+ levels. The accurate measurement of intracellular Ca2+ is, therefore, an important tool in neurotoxicology for assessing compounds/substances that disrupt Ca2+ homeostasis. Fluorescent, Ca2+-sensitive probes Indo-1 and Fluo-3 allow for the quantification of intracellular Ca2+ in individual cells to determine what effects neurotoxins have on both basal and stimulus-dependent Ca2+ concentrations.
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Acknowledgements
This work was supported by NIH Grants P30ES07033 and T32ES07032. The authors wish to express their sincere thanks to Dr. Michelle C. Catlin for helpful discussions.
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Cox, D.P., White, C.C., Kavanagh, T.J. (2011). Fluorescent Assessment of Intracellular Calcium in Individual Cells. In: Costa, L., Giordano, G., Guizzetti, M. (eds) In Vitro Neurotoxicology. Methods in Molecular Biology, vol 758. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-170-3_16
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DOI: https://doi.org/10.1007/978-1-61779-170-3_16
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