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In Vitro Neurotoxicology pp 215–235Cite as

Assessing Neuronal Bioenergetic Status

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 758))

Abstract

Drug discovery and therapeutic development for disorders of the central nervous system (CNS) represents one of the largest unmet markets in modern medicine. We have increasingly recognized that the lack of stringent assessment of mitochondrial function during the discovery process has resulted in drug recalls, black box warnings, and an urgent need to understand the metabolic liability of small molecules in neural systems. Given that the brain is the most energetically demanding organ, even modest perturbations in neuronal energetic pathways have been shown to impact growth, signaling, connectivity, and the restorative capacity of the CNS. In this work, we describe several tools to assess metabolic activity of primary neuronal cultures and neural cell lines using an acute model of injury induced by oxygen glucose deprivation. Methods include the measurement of total ATP and NADH, enzymatic assessment of lactate production by anaerobic respiration, as well as viability assays. We also present a modified screening method for assessing aerobic respiration of immortalized cell lines using galactose challenge.

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Author information

Authors and Affiliations

  1. Departments of Neurology and Pharmacology, School of Medicine, Vanderbilt University, Nashville, TN, USA

    BethAnn McLaughlin

Authors
  1. Stephanie L. H. Zeiger
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  2. Jeannette N. Stankowski
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  3. BethAnn McLaughlin
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Corresponding author

Correspondence to BethAnn McLaughlin .

Editor information

Editors and Affiliations

  1. , Department of Environmental and, University of Washington, Roosevelt Way NE 4225, Seattle, 98105, Washington, USA

    Lucio G. Costa

  2. , Department of Environmental and, University of Washington, Roosevelt Way NE 4225, Seattle, 98105, Washington, USA

    Gennaro Giordano

  3. Jesse Brown VA Medical Center, Department of Psychiatry, University of Illinois at Chicago, South Damen Avenue 820, Seattle, 60612, Illinois, USA

    Marina Guizzetti

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© 2011 Springer Science+Business Media, LLC

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Zeiger, S.L.H., Stankowski, J.N., McLaughlin, B. (2011). Assessing Neuronal Bioenergetic Status. In: Costa, L., Giordano, G., Guizzetti, M. (eds) In Vitro Neurotoxicology. Methods in Molecular Biology, vol 758. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-170-3_15

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  • DOI: https://doi.org/10.1007/978-1-61779-170-3_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-169-7

  • Online ISBN: 978-1-61779-170-3

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