Abstract
Integrins are heterodimeric membrane glycoproteins composed of noncovalently associated α and β subunits. Integrins support cell attachment and migration on the surrounding extracellular matrix as well as mediate cell–cell interaction in physiological and pathological settings. Constant recycling of integrins from the plasma membrane to the endosome makes integrins ideal receptors for the delivery of drugs to the cell cytoplasm. RNA interference (RNAi) has evolved not only as a powerful tool for studying gene expression and validating new drug targets, but also as a potential therapeutic intervention. However, the major challenge facing the translation of RNAi into clinical practice is the lack of efficient systemic delivery to specific cell types. Utilizing integrins as delivery target, we have recently devised a strategy to target leukocytes termed Integrin-targeted and stabilized NanoParticles (I-tsNPs) that entrap high RNAi payloads and deliver them in a leukocyte-specific manner to induce robust gene silencing.
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Acknowledgments
The authors would like to thank Prof. Motomu Shimaoka for his advice and encouragement and Dr. Yoshiyuki Morishita, and Dr. Charudharshini Srinivasan, for technical assistance. This work was supported by the European union – IRG (D.P.), the Alon Foundation (D.P.), and the Lewis Trust for Cancer Research (D.P.).
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Ben-Arie, N., Kedmi, R., Peer, D. (2011). Integrin-Targeted Nanoparticles for siRNA Delivery. In: Shimaoka, M. (eds) Integrin and Cell Adhesion Molecules. Methods in Molecular Biology, vol 757. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-166-6_29
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DOI: https://doi.org/10.1007/978-1-61779-166-6_29
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