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Laser-Capture Microdissection for Factor VIII-Expressing Endothelial Cells in Cancer Tissues

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Laser Capture Microdissection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 755))

Abstract

The immunostaining based Laser-capture microdissection (LCM) method called immune-LCM allows us to quantify the mRNA. Immune-LCM has recently been introduced to enhance identification of cells carrying a particular protein from frozen tissue samples. We have recently performed the immune-LCM of formaldehyde-fixated, paraffin-embedded tissues immunostained with a monoclonal antibody Factor VIII. This method could be useful for quantitative gene expression analysis in blood vessels from tumors of patients that have been treated with antiangiogenic drugs, allowing for validation of the effect of drug on the expected targets. Such capability might be exceedingly useful for the evaluation of the bioactivity of new drugs. This method is also useful to compare gene expression patterns in tumor cells versus endothelial cells during tumor progression or tumor angiogenesis.

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Authors and Affiliations

  1. Cariology, Operative Dentistry and Endodontics, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Niigata, Japan

    Tomoatsu Kaneko & Takashi Okiji

  2. Applied Molecular Medicine, Niigata University Graduate School of Medical and Dental Sciences, Chuo-Ku, Niigata, Japan

    Reika Kaneko

  3. Pulp Biology and Endodontics, Department of Restorative Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan

    Hideaki Suda

  4. Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, MI, USA

    Jacques E. Nör

  5. Department of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, MI, USA

    Jacques E. Nör

  6. Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI, USA

    Jacques E. Nör

Authors
  1. Tomoatsu Kaneko
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  2. Takashi Okiji
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  3. Reika Kaneko
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  4. Hideaki Suda
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  5. Jacques E. Nör
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Corresponding author

Correspondence to Tomoatsu Kaneko .

Editor information

Editors and Affiliations

  1. , Department of Pathology, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, United Kingdom

    Graeme I. Murray

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© 2011 Springer Science+Business Media, LLC

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Kaneko, T., Okiji, T., Kaneko, R., Suda, H., Nör, J.E. (2011). Laser-Capture Microdissection for Factor VIII-Expressing Endothelial Cells in Cancer Tissues. In: Murray, G. (eds) Laser Capture Microdissection. Methods in Molecular Biology, vol 755. Humana Press. https://doi.org/10.1007/978-1-61779-163-5_33

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  • DOI: https://doi.org/10.1007/978-1-61779-163-5_33

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-162-8

  • Online ISBN: 978-1-61779-163-5

  • eBook Packages: Springer Protocols

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