Detection and Characterization of Receptor Interactions with PDZ Domains
Many transmembrane receptors are regulated by associations with scaffold proteins containing PDZ domains, which interact with receptor carboxyl-termini to control receptor signaling, trafficking, and localization. We describe here approaches for detecting and characterizing interactions between receptors and PDZ scaffolds. These approaches include the construction and screening of proteomic arrays, blot overlays using fusion proteins, and co-immunoprecipitation studies to assess interactions in cells.
Key wordsG protein-coupled receptor PDZ domain Scaffold Affinity Growth factor Tyrosine kinase
The authors’ research is funded by the National Institutes of Health, USA.
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