Detection and Characterization of Receptor Interactions with PDZ Domains

  • Stefanie L. Ritter
  • Randy A. HallEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 756)


Many transmembrane receptors are regulated by associations with scaffold proteins containing PDZ domains, which interact with receptor carboxyl-termini to control receptor signaling, trafficking, and localization. We describe here approaches for detecting and characterizing interactions between receptors and PDZ scaffolds. These approaches include the construction and screening of proteomic arrays, blot overlays using fusion proteins, and co-immunoprecipitation studies to assess interactions in cells.

Key words

G protein-coupled receptor PDZ domain Scaffold Affinity Growth factor Tyrosine kinase 



The authors’ research is funded by the National Institutes of Health, USA.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of PharmacologyEmory University School of MedicineAtlantaUSA

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