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Visualizing G Protein-Coupled Receptor Signalsomes Using Confocal Immunofluorescence Microscopy

  • Sudha K. ShenoyEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 756)

Abstract

The heptahelical G protein-coupled receptors (GPCRs) receive and transmit a wide range of extracellular stimuli and induce a wide array of cellular responses by activating signaling kinases. It has become increasingly evident that the agonist-stimulated GPCR complexed with the adaptor protein, β-arrestin, serves as a focal point to recruit, activate, and target kinases to discrete subcellular compartments. This chapter describes a protocol to visualize the changes in the subcellular distribution of activated extracellular signal-regulated kinases 1 and 2 (ERK1/2) when induced by the angiotensin II type 1a receptor.

Key words

G protein-coupled receptor Confocal microscopy Extracellular signal-regulated kinase Arrestin Endocytosis Signaling Angiotensin G protein Scaffold 

Notes

Acknowledgments

This work was supported by National Institutes of Health grant HL 080525.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Departments of Medicine and Cell BiologyDuke University Medical CenterDurhamUSA

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