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Visualizing Receptor Endocytosis and Trafficking

  • Ali Salahpour
  • Larry S. BarakEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 756)

Abstract

G-protein-coupled 7 transmembrane domain receptors (GPCR-7TMR) represent the largest class of membrane protein drug targets. They respond to a plethora of ligands ranging from small molecules to polypeptide hormones. Upon activation, almost all GPCR-7TMRs undergo desensitization followed by receptor internalization and resensitization. This cycle is crucially important for regulating the signal emanating from the receptor and is tightly linked to the receptor and/or the ligands studied. In this chapter, we describe some of the technical approaches that can be used to study GPCR internalization and trafficking.

Key words

G-protein-coupled receptor Green fluorescent protein Immunofluorescence Internalization Trafficking 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Pharmacology and ToxicologyUniversity of TorontoTorontoCanada
  2. 2.Department of Cell BiologyDuke UniversityDurhamUSA

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