Using FRET-Based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity

  • Charlene Depry
  • Jin ZhangEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 756)


The ubiquitous Protein Kinase A (PKA) signaling pathway is responsible for the regulation of numerous processes including gene expression, metabolism, cell growth, and cell proliferation. This method details how to monitor real-time PKA activity dynamics in mammalian cells using fluorescence resonance energy transfer (FRET)-based reporters.

Key words

Protein kinase A Kinase activity reporter Fluorescence resonance energy transfer Live-cell imaging 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Pharmacology and Molecular SciencesThe Johns Hopkins University School of MedicineBaltimoreUSA
  2. 2.Solomon H. Snyder Department of NeuroscienceThe Johns Hopkins University School of MedicineBaltimoreUSA
  3. 3.Department of OncologyThe Johns Hopkins University School of MedicineBaltimoreUSA

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