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Using FRET-Based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity

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Signal Transduction Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 756))

Abstract

The ubiquitous Protein Kinase A (PKA) signaling pathway is responsible for the regulation of numerous processes including gene expression, metabolism, cell growth, and cell proliferation. This method details how to monitor real-time PKA activity dynamics in mammalian cells using fluorescence resonance energy transfer (FRET)-based reporters.

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Author information

Authors and Affiliations

  1. Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Charlene Depry & Jin Zhang

  2. Solomon H. Snyder Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Jin Zhang

  3. Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Jin Zhang

Authors
  1. Charlene Depry
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  2. Jin Zhang
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Corresponding author

Correspondence to Jin Zhang .

Editor information

Editors and Affiliations

  1. Div. Endocrinology, Metabolism &, Medical Genetics, Medical University of South Carolina, Jonathan Lucas Street 96, Charleston, 29425, South Carolina, USA

    Louis M. Luttrell

  2. Dept. Physiology & Pharmacology, University of Western Ontario, London, N6A 5C1, Ontario, Canada

    Stephen S. G. Ferguson

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Depry, C., Zhang, J. (2011). Using FRET-Based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity. In: Luttrell, L., Ferguson, S. (eds) Signal Transduction Protocols. Methods in Molecular Biology, vol 756. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-160-4_16

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  • DOI: https://doi.org/10.1007/978-1-61779-160-4_16

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-159-8

  • Online ISBN: 978-1-61779-160-4

  • eBook Packages: Springer Protocols

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