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Analysis of GPCR/Ion Channel Interactions

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Signal Transduction Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 756))

Abstract

Voltage-gated calcium channels are key regulators of calcium homeostasis in excitable cells. A number of cellular signaling pathways serve to fine tune calcium channel activity, including the activation of G protein-coupled receptors. Besides regulating channel activity via second messengers, GPCRs can also physically associate with calcium channels to directly regulate their functions, as well as their trafficking to and from the plasma membrane. Here we provide some methods that can be used to examine channel–receptor interactions and co-trafficking. While we focus on voltage-gated calcium channels, the techniques described herein are broadly applicable to other types of channels.

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Acknowledgments

We thank Dr. Bourinet for creating an HA-tagged version of the Cav2.2 channel. GWZ is Scientist of the Alberta Heritage Foundation for Medical Research, and a Canada Research Chair. Work described in this chapter was supported by grants from the Canadian Institutes of Health Research.

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Authors and Affiliations

  1. Department of Physiology and Pharmacology, Hotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada

    Christophe Altier & Gerald W. Zamponi

Authors
  1. Christophe Altier
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  2. Gerald W. Zamponi
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Corresponding author

Correspondence to Gerald W. Zamponi .

Editor information

Editors and Affiliations

  1. Div. Endocrinology, Metabolism &, Medical Genetics, Medical University of South Carolina, Jonathan Lucas Street 96, Charleston, 29425, South Carolina, USA

    Louis M. Luttrell

  2. Dept. Physiology & Pharmacology, University of Western Ontario, London, N6A 5C1, Ontario, Canada

    Stephen S. G. Ferguson

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Altier, C., Zamponi, G.W. (2011). Analysis of GPCR/Ion Channel Interactions. In: Luttrell, L., Ferguson, S. (eds) Signal Transduction Protocols. Methods in Molecular Biology, vol 756. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-160-4_11

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  • DOI: https://doi.org/10.1007/978-1-61779-160-4_11

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-159-8

  • Online ISBN: 978-1-61779-160-4

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