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A Transposon-Based Activation Tagging System for Gene Function Discovery in Arabidopsis

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Plant Transcription Factors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 754))

Abstract

Activation tagging is a powerful strategy to find new gene functions, especially from genes that are redundant or show lethal knock-out phenotypes. It has been applied using T-DNA or transposons. En/Spm-I/dSpm engineered transposons are efficient activation tags in Arabidopsis. An immobilized transposase source and an enhancer-bearing non-autonomous element are used in combination with positive and negative selectable markers to generate a population of single- or low-copy, stable insertions. This method describes the steps required for selection of parental lines, generation of a population of stable insertions, and gene identification.

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Authors and Affiliations

  1. Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Irapuato, Guanajuato, México

    Nayelli Marsch-Martínez

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  1. Nayelli Marsch-Martínez
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Correspondence to Nayelli Marsch-Martínez .

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  1. College of Agriculture, Dept. Plant & Soil Sciences, University of Kentucky, University Drive 1401, Lexington, 40546-0236, Kentucky, USA

    Ling Yuan

  2. Dept. Plant & Soil Sciences, University of Kentucky, Veterans Dr. 1405, Lexington, 40546-0312, Kentucky, USA

    Sharyn E. Perry

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Marsch-Martínez, N. (2011). A Transposon-Based Activation Tagging System for Gene Function Discovery in Arabidopsis . In: Yuan, L., Perry, S. (eds) Plant Transcription Factors. Methods in Molecular Biology, vol 754. Humana Press. https://doi.org/10.1007/978-1-61779-154-3_4

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  • DOI: https://doi.org/10.1007/978-1-61779-154-3_4

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