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Preparation and Dynamic Patterning of Supported Lipid Membranes Mimicking Cell Membranes

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Bioconjugation Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 751))

Abstract

In this chapter, we describe standardized protocols for the self-assembly of supported lipid bilayers (SLBs) from liposomes with lipid compositions mimicking eukaryote and prokaryote cell membranes. Such SLBs can also contain lipids with polymeric and glycosylated headgroups. Furthermore, we present protocols on how to manipulate the adsorption and desorption of membranes on indium tin oxide (ITO) ­electrodes, which allows for the creation of patterned and in situ regenerated SLB arrays that can be used to study electrochemically mediated membrane processes in a microarray format.

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Authors and Affiliations

  1. Laboratory for Surface Science and Technology, Swiss Federal Institute of Technology Zurich (ETH Zurich), Zurich, Switzerland

    Erik Reimhult

Authors
  1. Stefan Kaufmann
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  2. Karthik Kumar
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  3. Erik Reimhult
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Corresponding author

Correspondence to Erik Reimhult .

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Editors and Affiliations

  1. Pacific Biosciences, Willow Road 1380, Menlo Park, 94025, California, USA

    Sonny S. Mark

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Kaufmann, S., Kumar, K., Reimhult, E. (2011). Preparation and Dynamic Patterning of Supported Lipid Membranes Mimicking Cell Membranes. In: Mark, S. (eds) Bioconjugation Protocols. Methods in Molecular Biology, vol 751. Humana Press. https://doi.org/10.1007/978-1-61779-151-2_28

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  • DOI: https://doi.org/10.1007/978-1-61779-151-2_28

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-150-5

  • Online ISBN: 978-1-61779-151-2

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