Abstract
How cells interact with the extracellular matrix (ECM) is important for a number of fundamental processes in cell biology. However, the ECM is highly complex and in order to simplify the matrix for cell biological studies, it has been modeled with self-assembled monolayers (SAMs) of alkanethiolates on gold substrates. In this chapter, we outline procedures to create dynamic surfaces by functionalizing SAMs. SAMs based on quinone, oxyamine, and alcohol-terminated thiols were used to immobilize cell adhesive peptides with spatial control. Cells were seeded to these surfaces to provide cell co-culture patterns suitable for biological studies.
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Acknowledgments
This work was supported by the Carolina Center for Cancer Nanotechnology Excellence (NCI), and by grants from the Burroughs Wellcome Foundation (Interface Career Award) and the National Science Foundation (Career Award) to M.N.Y.
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Westcott, N.P., Yousaf, M.N. (2011). Fabrication of Dynamic Self-Assembled Monolayers for Cell Migration and Adhesion Studies. In: Mark, S. (eds) Bioconjugation Protocols. Methods in Molecular Biology, vol 751. Humana Press. https://doi.org/10.1007/978-1-61779-151-2_26
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DOI: https://doi.org/10.1007/978-1-61779-151-2_26
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