Abstract
The ability to examine cells tagged with fluorescent proteins in vivo has led to exciting advances in molecular neurobiology. The integral role of Schwann cells in nerve regeneration is well characterized, but not until recently has dynamic imaging of these critical cells been possible. Unlike many static techniques, in vivo imaging of Schwann cells tagged with green fluorescent protein (GFP) allows for serial imaging and may provide important insights into their role in peripheral nerve regeneration. In this chapter, we describe a protocol for in vivo imaging of SC migration into nonfluorescent nerve grafts using transgenic S100-GFP mice.
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References
Lichtman, J.W., and Conchello, J.A. (2005) Fluorescence microscopy Nat Methods 2, 910–9.
Hayashi, A., Koob, J.W., Liu, D.Z., et al. (2007) A double-transgenic mouse used to track migrating Schwann cells and regenerating axons following Âengraftment of injured nerves Exp Neurol 207, 128–38.
Misgeld, T., and Kerschensteiner, M. (2006) In vivo imaging of the diseased nervous Âsystem Nat Rev Neurosci 7, 449–63.
Bhatheja, K., and Field, J. (2006) Schwann cells: origins and role in axonal maintenance and regeneration Int J Biochem Cell Biol 38, 1995–9.
Bunge, R.P. (1994) The role of the Schwann cell in trophic support and regeneration J Neurol 242, S19–21.
Zuo, Y., Lubischer, J.L., and Kang, H., et al. (2004) Fluorescent proteins expressed in mouse transgenic lines mark subsets of glia, neurons, macrophages, and dendritic cells for vital examination J Neurosci 24, 10999–1009.
Misgeld, T., Nikic, I., and Kerschensteiner, M. (2007) In vivo imaging of single axons in the mouse spinal cord Nat Protoc 2, 263–8.
Keller-Peck, C.R., Walsh, M.K., Gan, W.B., Feng, G., Sanes, J.R., and Lichtman, J.W. (2001) Asynchronous synapse elimination in neonatal motor units: studies using GFP transgenic mice Neuron 31, 381–94.
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Kasukurthi, R., Myckatyn, T.M. (2011). Imaging of Schwann Cells In Vivo. In: Filippi, MD., Geiger, H. (eds) Stem Cell Migration. Methods in Molecular Biology, vol 750. Humana Press. https://doi.org/10.1007/978-1-61779-145-1_6
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DOI: https://doi.org/10.1007/978-1-61779-145-1_6
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Online ISBN: 978-1-61779-145-1
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