Abstract
Adult neural progenitors originating is the subventricular zone (SVZ) proceed toward the olfactory bulb (OB) along a peculiar path of migration known as the rostral migratory stream. Once reaching the inner core of the bulb, neuroblasts migrate radially to various layers of the adult OB where they differentiate into local interneurons. We recently demonstrated that this latter form of migration is guided by blood vessels that serve as a scaffold for neural progenitor migration to their respective OB layers. Migration of progenitors along blood vessels can be visualized by time-lapse confocal imaging, which requires labeling of both migrating cells and blood vessels. In this article, we describe procedures to label neural progenitors in adult through in vivo stereotaxic cell tracer injection into the SVZ, and to visualize blood vessels through luminal filling with stabilized fluorescent dyes. Moreover, we illustrate the methods for acute brain slices preparation for imaging and procedures used during confocal time-lapse imaging to follow the movement of adult neural progenitors along blood vessels.
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References
Gasser, U.E., and Hatten, M.E. (1990) Central nervous system neurons migrate on astroglial fibers from heterotypic brain regions in vitro Proc Natl Acad Sci USA 87, 4543–47.
Wichterle, H, Garcia-Verdugo, J.M., and varez-Buylla, A. (1997) Direct evidence for homotypic, glia-independent neuronal migration Neuron 1997 18, 779–91.
Lois, C., Garcia-Verdugo, J.M., and Alvarez-Buylla, A. (1996) Chain migration of neuronal precursors Science 1996 271, 978–81.
Bovetti, S., Hsieh, Y.C., Bovolin, P., Perroteau, I., Kazunori, T., and Puche, A.C. (2007) Blood vessels form a scaffold for neuroblast migration in the adult olfactory bulb J Neurosci 27, 5976–80.
Snapyan, M., Lemasson, M., Brill, M.S., Blais, M., Massouh, M., Ninkovic, J., Gravel, C., Berthod, F., Gotz, M., Barker, P.A., Parent, A., and Saghatelyan, A. (2009)Vasculature guides migrating neuronal precursors in the adult mammalian forebrain via brain-derived neurotrophic factor signaling J Neurosci 29, 4172–88.
Honda, S., Toda, K., Tozuka, Y., Yasuzawa, S., Iwabuchi, K., and Tomooka, Y. (2007) Migration and differentiation of neural cell lines transplanted into mouse brains Neurosci Res. 59, 124–35.
Ohab, J.J., and Carmichael, S.T. (2008) Poststroke neurogenesis: emerging principles of migration and localization of immature neurons Neuroscientist 14, 369–380.
Atho,s J., and Storm, D.R. (2001) High precision stereotaxic surgery in mice Curr Protoc Neurosci Appendix 4: Appendix.
De Marchis, S., Fasolo, A., Shipley, M., and Puche, A. (2001) Unique neuronal tracers show migration and differentiation of SVZ progenitors in organotypic slices J Neurobiol 49, 326–38.
Petreanu, L., and Alvarez-Buylla, A. (2002) Maturation and death of adult-born olfactory bulb granule neurons: role of olfaction J Neurosci 22, 6106–13.
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Puche, A.C., Bovetti, S. (2011). Studies of Adult Neural Stem Cell Migration. In: Filippi, MD., Geiger, H. (eds) Stem Cell Migration. Methods in Molecular Biology, vol 750. Humana Press. https://doi.org/10.1007/978-1-61779-145-1_16
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DOI: https://doi.org/10.1007/978-1-61779-145-1_16
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