Abstract
Recent advances in cell biology have demonstrated the role of multiple signaling proteins in the transduction of external signals to cytoplasmic and nuclear effectors, controlling the movement and/or retention of hematopoietic stem cells and progenitors (HSC/P) within the bone marrow, with important clinical implications. Multiple assays have become routine in the analysis of adhesion to the microenvironment, migration toward chemoattractant gradients, and homing of HSC/P in the bone marrow in vivo. In this chapter, we analyze some of the most frequently used assays in our laboratory to explore the ability of HSC to migrate, adhere, and home in in vitro and in vivo assays.
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Acknowledgments
The author wishes to acknowledge the contributions to these protocols by all the co-authors of his publications, and thanks Margaret O’Leary for her editing assistance. The author wishes to thank Margaret O’Leary for helpful edition. This work has been supported by National Institutes of Health (1R01HL08-7159 and supplement 3R01HL087159), Department of Defense (CM-064050), Heimlich Institute Foundation, Alex’s Lemonade Stand Foundation, and National Blood Foundation.
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Cancelas, J.A. (2011). Adhesion, Migration, and Homing of Murine Hematopoietic Stem Cells and Progenitors. In: Filippi, MD., Geiger, H. (eds) Stem Cell Migration. Methods in Molecular Biology, vol 750. Humana Press. https://doi.org/10.1007/978-1-61779-145-1_13
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DOI: https://doi.org/10.1007/978-1-61779-145-1_13
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