Abstract
Compared with other vertebrate animal models, zebrafish (Danio rerio) has its superior advantages for studying stem cell migration. Zebrafish have similar tissues and organs as mammals, where tissue-specific stem cells reside in. Zebrafish eggs are externally fertilized and remain transparent until most of the organs are fully developed. This allows imaging stem cells in vivo very easily. Recently, a zebrafish double pigmentation mutant, casper, became a new popular imaging model in the zebrafish field due to its completely transparent bodies in adulthood. It has been used as an excellent model to study adult hematopoietic stem cell (HSC) in the transplantation setting. The unparalleled imaging power of zebrafish provides great opportunities of tracing stem cells in vivo in the developmental and regenerative context. In this chapter, we use HSC as an example and combine the powerful imaging techniques in zebrafish, to provide protocols for in vivo imaging fluorescence-labeled stem cell migration, stem cell fate tracing in zebrafish embryos, HSC transplantation, and in vivo imaging in both zebrafish embryos and adults. These techniques can also be applied to other types of stem cells in zebrafish embryos and adults.
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References
de Jong, J.L., and Zon, L.I. (2005) Use of the zebrafish system to study primitive and definitive hematopoiesis Annu Rev Genet 39, 481–501.
North, T.E., Goessling, W., Walkley, C.R., et al. (2007) Prostaglandin E2 regulates vertebrate haematopoietic stem cell homeostasis Nature 447, 1007–11.
Dorsky, R.I., Moon, R.T., and Raible, D.W. (1998) Control of neural crest cell fate by the Wnt signalling pathway Nature 396, 370–3.
White, R.M., and Zon, L.I. (2008) Melanocytes in development, regeneration, and cancer Cell Stem Cell 3, 242–52.
Chapouton, P., Adolf, B., Leucht, C., et al. (2006) her5 expression reveals a pool of neural stem cells in the adult zebrafish midbrain Development 133, 4293–303.
Stigloher, C., Chapouto, P., Adolf, B., and Bally-Cuif, L. (2008) Identification of neural progenitor pools by E(Spl) factors in the embryonic and adult brain Brain Res Bull 75, 266–73.
Johnson, S.L., and Bennett, P. (1999) Growth control in the ontogenetic and regenerating zebrafish fin Methods Cell Biol 59, 301–11.
White, R.M., Sessa, A., and Burke, C., et al. (2008) Transparent adult zebrafish as a tool for in vivo transplantation analysis Cell Stem Cell 2, 183–9.
Galloway, J.L., and Zon, L.I. (2003) Ontogeny of hematopoiesis: examining the emergence of hematopoietic cells in the vertebrate embryo Curr Top Dev Biol 53, 139–58.
Kalev-Zylinska, M.L., Horsfield, J.A., Flores, M.V., et al. (2002) Runx1 is required for zebrafish blood and vessel development and expression of a human RUNX1-CBF2T1 transgene advances a model for studies of leukemogenesis Development 129, 2015–30.
Lam, E.Y., Chau, J.Y., Kalev-Zylinska, M.L., et al. (2008) Zebrafish runx1 promoter-EGFP transgenics mark discrete sites of definitive blood progenitors Blood 113, 1241–1249.
Bertrand, J.Y., Kim, A.D., Teng, S., and Traver, D. (2008) CD41+ cmyb + precursors colonize the zebrafish pronephros by a novel migration route to initiate adult hematopoiesis Development 135, 1853–62.
Mucenski, M.L., McLain, K., Kier, A.B., et al. (1991) A functional c-myb gene is required for normal murine fetal hepatic hematopoiesis Cell 65, 677–89.
Lin, H.F., Traver, D., Zhu, H., et al. (2005) Analysis of thrombocyte development in CD41-GFP transgenic zebrafish Blood 106, 3803–10.
Traver, D., Winzeler, A., Stern, H.M., et al. (2004) Effects of lethal irradiation in zebrafish and rescue by hematopoietic cell transplantation Blood 104, 1298–305.
Monte Westerfield IoN, University of Oregon. 2000 The Zebrafish Book. 4 ed: University of Oregon Press.
Traver, D., Paw, B.H., Poss, K.D., Penberthy, W.T., Lin, S., and Zon, L.I. (2003) Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants Nat Immunol 4, 1238–46.
Zhu, H., Traver D., Davidson, A.J., et al. (2005) Regulation of the lmo2 promoter during hematopoietic and vascular development in zebrafish Dev Biol 281, 256–69.
Gillette-Ferguson, I., Ferguson, D.G., Poss, K.D., and Moorman, S.J. (2003) Changes in gravitational force induce alterations in gene expression that can be monitored in the live, developing zebrafish heart Adv Space Res 32, 1641–6.
Lin, S. (2000) Transgenic zebrafish Methods Mol Biol 136, 375–83.
Rosen, J.N., Sweeney, M.F., and Mably, J.D. (2009) Microinjection of zebrafish embryos to analyze gene function J Vis Exp.
Kissa, K., Murayama, E., Zapata, A., et al. (2008) Live imaging of emerging hematopoietic stem cells and early thymus colonization Blood 111, 1147–56.
Bertrand, J.Y., Kim, A.D., Violette, E.P., Stachura, D.L., Cisson, J.L., and Traver, D. (2007) Definitive hematopoiesis initiates through a committed erythromyeloid progenitor in the zebrafish embryo Development 134, 4147–56.
Murayama, E., Kissa, K., Zapata, A., et al. (2006) Tracing hematopoietic precursor migration to successive hematopoietic organs during zebrafish development Immunity 25, 963–75.
Hatta, K., Tsujii, H., and Omura, T. (2006) Cell tracking using a photoconvertible fluorescent protein Nat Protoc 1, 960–7.
Acknowledgments
We thank Dr. Owen Tamplin for reading the manuscript, Dr. Richard M. White for developing the zebrafish retro-orbital injection technique, and the rest of the Zon lab for the constant advice and help.
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Li, P., Zon, L.I. (2011). Stem Cell Migration: A Zebrafish Model. In: Filippi, MD., Geiger, H. (eds) Stem Cell Migration. Methods in Molecular Biology, vol 750. Humana Press. https://doi.org/10.1007/978-1-61779-145-1_11
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DOI: https://doi.org/10.1007/978-1-61779-145-1_11
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