Mapping of Crossover Sites Using DNA Microarrays
Crossovers (COs) play an essential role in promoting successful chromosome segregation during meiosis. Crossing over generates chiasmata, which are physical bridges between homologs that provide the appropriate tension to properly align chromosomes on the meiosis I spindle. Homolog pairs that fail to cross over can result in meiosis I nondisjunction, leading to aneuploid gametes. Therefore, the number and distribution of crossovers are tightly regulated to ensure that each chromosome pair receives at least one CO. Here, we describe a DNA microarray-based method to map CO distribution genome-wide, on a cell-by-cell basis, allowing for rapid and accurate analysis of multiple aspects of CO control.
Key wordsMeiosis, recombination crossover noncrossover direct allelic scanning crossover interference crossover homeostasis S96 YJM789
We thank Carol Anderson for optimizing an alternative yeast inoculation method for genomic DNA extraction. We thank Ashwini Oke for her assistance in performing the DNase I digestion for this publication. We also thank Mike Pollard for critical reading of the manuscript. S.Y.C. is supported by a Genentech Fellowship. J.C.F. is supported by the American Cancer Society Research Scholar Award (RSG CCG 110688).
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