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Mapping of Crossover Sites Using DNA Microarrays

  • Stacy Y. Chen
  • Jennifer C. FungEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 745)

Abstract

Crossovers (COs) play an essential role in promoting successful chromosome segregation during meiosis. Crossing over generates chiasmata, which are physical bridges between homologs that provide the appropriate tension to properly align chromosomes on the meiosis I spindle. Homolog pairs that fail to cross over can result in meiosis I nondisjunction, leading to aneuploid gametes. Therefore, the number and distribution of crossovers are tightly regulated to ensure that each chromosome pair receives at least one CO. Here, we describe a DNA microarray-based method to map CO distribution genome-wide, on a cell-by-cell basis, allowing for rapid and accurate analysis of multiple aspects of CO control.

Key words

Meiosis, recombination crossover noncrossover direct allelic scanning crossover interference crossover homeostasis S96 YJM789 

Notes

Acknowledgments

We thank Carol Anderson for optimizing an alternative yeast inoculation method for genomic DNA extraction. We thank Ashwini Oke for her assistance in performing the DNase I digestion for this publication. We also thank Mike Pollard for critical reading of the manuscript. S.Y.C. is supported by a Genentech Fellowship. J.C.F. is supported by the American Cancer Society Research Scholar Award (RSG CCG 110688).

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Obstetrics, Gynecology, and Reproductive SciencesUniversity of CaliforniaSan FranciscoUSA

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