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Studying DNA Replication Fork Stability in Xenopus Egg Extract

  • Yoshitami Hashimoto
  • Vincenzo CostanzoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 745)

Abstract

A crucial process to ensure cell survival and genome stability is the correct replication of the genome. DNA replication relies on complex machinery whose mechanisms are being elucidated using different model systems. A major aspect of this process, which is an intense subject of investigation, is what happens when replication forks encounter obstacles impairing their progression such as modified bases, pausing sites, and single strand breaks. The detailed biochemical analysis of DNA replication in the presence of DNA damage has been impeded by the lack of a cell-free system recapitulating DNA replication. Here we describe assays based on the vertebrate Xenopus laevis egg extract to study the biochemical aspects of replication fork stability.

Key words

DNA replication Xenopus laevis DNA repair DNA damage fork restart replication inhibitors and chromatin 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Clare Hall LaboratoriesLondon Research InstituteHertsfordshireUK

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