SNP-Based Mapping of Crossover Recombination in Caenorhabditis elegans

  • Grace C. Bazan
  • Kenneth J. HillersEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 745)


Caenorhabditis elegans is an important experimental organism for the study of recombination during meiosis. Here, we provide methods for the use of single-nucleotide polymorphisms (SNPs) for the study of crossing over in C. elegans.

Key words

Crossing over recombination PCR snip-SNP 



Anne Villeneuve helped with the preparation of a previous version of this manuscript. K.J.H. was supported by Award Number R15HD059093 from the Eunice Kennedy Shriver National Institute of Child Health and Human Development.


  1. 1.
    Wicks, S.R., Yeh, R.T., Gish, W.R., Waterston, R.H., and Plasterk, R.H. (2001) Rapid gene mapping in Caenorhabditis elegans using a high density polymorphism map. Nat Genet 28, 160–164.PubMedCrossRefGoogle Scholar
  2. 2.
    Hillers, K.J., and Villeneuve, A.M. (2009) Analysis of meiotic recombination in Caenorhabditis elegans. Methods Mol Biol 557, 77–97.PubMedCrossRefGoogle Scholar
  3. 3.
    Hillers, K.J., and Villeneuve, A.M. (2003) Chromosome-wide control of meiotic crossing over in C. elegans. Curr Biol 13, 1641–1647.PubMedCrossRefGoogle Scholar
  4. 4.
    Davis, M.W., and Hammarlund, M. (2006) Single-nucleotide polymorphism mapping. Methods Mol Biol 351, 75–92.PubMedGoogle Scholar
  5. 5.
    Carlton, P.M., Farruggio, A.P., and Dernburg, A.F. (2006) A link between meiotic progression and crossover control. PLoS Genet 2, e12.PubMedCrossRefGoogle Scholar
  6. 6.
    Lim, J.G., Stine, R.R., and Yanowitz, J.L. (2008) Domain-specific regulation of recombination in Caenorhabditis elegans in response to temperature, age and sex. Genetics 180, 715–726.PubMedCrossRefGoogle Scholar
  7. 7.
    Mets, D.G., and Meyer, B.J. (2009) Condensins regulate meiotic DNA break distribution, thus crossover frequency, by controlling chromosome structure. Cell 139, 73–86.PubMedCrossRefGoogle Scholar
  8. 8.
    Nabeshima, K., Villeneuve, A.M., and Hillers, K.J. (2004) Chromosome-wide regulation of meiotic crossover formation in Caenorhabditis elegans requires properly assembled chromosome axes. Genetics 168, 1275–1292.PubMedCrossRefGoogle Scholar
  9. 9.
    Saito, T.T., Youds, J.L., Boulton, S.J., and Colaiacovo, M.P. (2009) Caenorhabditis elegans HIM-18/SLX-4 interacts with SLX-1 and XPF-1 and maintains genomic integrity in the germline by processing recombination intermediates. PLoS Genet 5, e1000735.PubMedCrossRefGoogle Scholar
  10. 10.
    Tsai, C.J., Mets, D.G., Albrecht, M.R., Nix, P., Chan, A., and Meyer, B.J. (2008) Meiotic crossover number and distribution are regulated by a dosage compensation protein that resembles a condensin subunit. Genes Dev 22, 194–211.PubMedCrossRefGoogle Scholar
  11. 11.
    Hammarlund, M., Davis, M.W., Nguyen, H., Dayton, D., and Jorgensen, E.M. (2005) Heterozygous insertions alter crossover distribution but allow crossover interference in Caenorhabditis elegans. Genetics 171, 1047–1056.PubMedCrossRefGoogle Scholar
  12. 12.
    Rockman, M.V., and Kruglyak, L. (2009) Recombinational landscape and population genomics of Caenorhabditis elegans. PLoS Genet 5, e1000419.PubMedCrossRefGoogle Scholar
  13. 13.
    Davis, M.W., Hammarlund, M., Harrach, T., Hullett, P., Olsen, S., and Jorgensen, E.M. (2005) Rapid single nucleotide polymorphism mapping in C. elegans. BMC Genomics 6, 118.PubMedCrossRefGoogle Scholar
  14. 14.
    Brenner, S. (1974) The genetics of Caenorhabditis elegans. Genetics 77, 71–94.PubMedGoogle Scholar
  15. 15.
    McKim, K.S., Howell, A.M., and Rose, A.M. (1988) The effects of translocations on recombination frequency in Caenorhabditis elegans. Genetics 120, 987–1001.PubMedGoogle Scholar
  16. 16.
    MacQueen, A.J., Phillips, C.M., Bhalla, N., Weiser, P., Villeneuve, A.M., and Dernburg, A.F. (2005) Chromosome sites play dual roles to establish homologous synapsis during meiosis in C. elegans. Cell 123, 1037–1050.PubMedCrossRefGoogle Scholar
  17. 17.
    Edgley, M.L., Baillie, D.L., Riddle, D.L., and Rose, A.M. (April 6, 2006) Genetic balancers In WormBook, The C. elegans Research Community, WormBook, ed. doi/10.1895/wormbook.1.89.1,
  18. 18.
    Kelly, K.O., Dernburg, A.F., Stanfield, G.M., and Villeneuve, A.M. (2000) Caenorhabditis elegans msh-5 is required for both normal and radiation-induced meiotic crossing over but not for completion of meiosis. Genetics 156, 617–630.PubMedGoogle Scholar
  19. 19.
    Fire, A., Xu, S., Montgomery, M.K., Kostas, S.A., Driver, S.E., and Mello, C.C. (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806–811.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Biological SciencesCalifornia Polytechnic State UniversitySan Luis ObispoUSA

Personalised recommendations