Abstract
RNA interference (RNAi) is a useful research tool for the specific deletion, or knockdown, of target genes that can be exploited both in cultured plant cells and in whole plants. In RNAi, hairpin RNA (hpRNA)-transduced lines are used to identify loss-of-function mutations in multi-copy genes with redundant functions in polyploid plant species. Plants transformed with hpRNA exhibit a range of phenotypes resulting from complete knockdown to weak suppression or tissue- and stage-specific knockdown. Functional genomics using proteomic analysis with two-dimensional gel electrophoresis (2-DE) and mass spectrometry provides valuable information about altered levels of expression of specific genes in biological samples. Here, we describe the proteomic analysis of Oryza sativa (Os) thioredoxin m (Ostrxm) knockdown using 2-DE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
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Acknowledgments
This work was supported by grants from MEST/NRF to the WCU program (R32-10148), CFGC (CG3313-1) and NRL (M10600000205-06J0000-20510), and Technology Development Program for Agriculture and Forestry (609004-5) of ARPC, MIFAFF, Korea.
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Lee, S.Y., Lee, K.O. (2011). Proteomic Analysis of RNA Interference Induced Knockdown Plant. In: Kodama, H., Komamine, A. (eds) RNAi and Plant Gene Function Analysis. Methods in Molecular Biology, vol 744. Humana Press. https://doi.org/10.1007/978-1-61779-123-9_15
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DOI: https://doi.org/10.1007/978-1-61779-123-9_15
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