Abstract
The major phenotype of CF is the accumulation of mucus, a phenomenon whose relation to the dysfunctional CFTR is still not fully understood. This means that studies of mucus and its main component, the mucins, are important. Due to the large size and high glycosylation level, such questions need special considerations and methodology. We describe methods for the general quantification of heavily glycosylated proteins as the mucins using dot/slot blot. We also describe the separation of the mucins by gel electrophoresis and the identification with specific antibodies on Western blot and by proteomics.
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Acknowledgments
This work was supported by the Swedish Research Council (no. 7461, 21027, and 342-2004-4434), The Swedish Cancer Foundation, The Knut and Alice Wallenberg Foundation (KAW2007.0118), IngaBritt and Arne Lundberg Foundation, Sahlgren’s University Hospital (LUA-ALF), EU-FP7 IBDase, Wilhelm and Martina Lundgren’s Foundation, Söderbergs Stiftelser, Swedish CF Foundation, and The Swedish Foundation for Strategic Research – Innate Immunity, and The Mucosal Immunobiology and Vaccine Center (MIVAC).
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Thomsson, K.A., Hansson, G.C. (2011). Identification and Quantification of Mucin Expression. In: Amaral, M., Kunzelmann, K. (eds) Cystic Fibrosis. Methods in Molecular Biology, vol 742. Humana Press. https://doi.org/10.1007/978-1-61779-120-8_8
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DOI: https://doi.org/10.1007/978-1-61779-120-8_8
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