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Cloning Small RNAs

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RNA Interference Techniques

Part of the book series: Neuromethods ((NM,volume 58))

Abstract

Small, noncoding RNAs have proven to be powerful and ubiquitous agents of gene regulation in eukaryotic genomes. Since their initial discovery, several methods have been developed for directly cloning and sequencing these tiny RNA species. These cloning methods are presented along with a discussion of ways to enhance cloning efficiency and success. In addition, major advances in massively parallel, next-generation sequencing methods are presented as they impact small RNA studies. Finally, results of the application of one of the extant in vitro cloning methods to a marsupial mammal model are presented.

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Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, University of Iowa Carver College of Medicine, Iowa City, IA, USA

    Eric J. Devor

Authors
  1. Eric J. Devor
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  2. Lingyan Huang
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Corresponding author

Correspondence to Eric J. Devor .

Editor information

Editors and Affiliations

  1. Research Institute, Center for Gene Therapy, Nationwide Children’s Hospital, Children's Drive 700, Columbus, 43205, Ohio, USA

    Scott Q. Harper

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Devor, E.J., Huang, L. (2011). Cloning Small RNAs. In: Harper, S. (eds) RNA Interference Techniques. Neuromethods, vol 58. Humana Press. https://doi.org/10.1007/978-1-61779-114-7_5

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  • DOI: https://doi.org/10.1007/978-1-61779-114-7_5

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-113-0

  • Online ISBN: 978-1-61779-114-7

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