Abstract
Phospholipidomics by high-performance liquid chromatography coupled to electrospray mass spectrometry or tandem mass spectrometry, including sample preparation, instrumental analyses, and data interpretation, is described in the chapter.
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References
Hsu FF, Turk J. (2003) Electrospray ionization/tandem quadrupole mass spectrometric studies on phosphatidylcholine: The fragmentation processes. J. Am. Soc. Mass Spectrom. 14:352–63.
Chen S, Curcuruto O, et al. Identification of phospholipid molecular species containing two fatty acyl chains differing by 2D by negative-ion fast atom bombardment with mass-analyzed ion kinetic energy analysis. Rapid Communi. Mass Spectrom. 1992;6:454–58.
Hsu FF, Turk J, et al. Characterization of alkylacyl, alk-1-enylacyl and lysosubclasses of glycerophosphocholine by tandem quadrupole mass spectrometry with electrospray Âionization. J. Mass Spectrom. 2003;38:752–63.
Han X, Gross, R.W. Structural determination of lysophospholipid regioisomers by electrospray ionization tandem mass spectrometry. J. Am. Chem. Soc. 1996;118:451–57.
Chen S, Li, K.W. Mass spectrometric identification of molecular species of phosphatidylcholine and Lysophosphatidylcholine extracted from shark liver. J. Agric. Food Chem. 2007;55:9670–77.
Chen S. Tandem mass spectrometric approach for determining structure of molecular species of aminophospholipids. Lipids, 1997;32:85–100.
Hsu FF, Turk J. Charge-remote and charge-driven fragmentation processes in diacyl glycerophosphoethanolamine upon low-energy collisional activation: a mechanistic proposal. J. Am. Soc. Mass Spectrom. 2000;11:892–99.
Chen S, Carvey PM, Li KW. Characterization of the molecular species of phosphatidylethanolamine from kidney of fresh water snail lymneae stagnalis by mass spectrometry. Rapid Commun. Mass Spectrom. 1999;13:2416–23.
Chen S, Li KW. Comparison of molecular species of various transphosphatidylated phosphatidylserine (PS) with bovine cortex PS by mass spectrometry. Chem. Phys. Lipids 2008;152:46–56.
Hsu FF, Turk J. Studies on phosphatidylserine by tandem quadrupole and multiple stage quadrupole ion-trap mass spectrometry with electrospray ionization: structural characterization and the fragmentation processes. J. Am. Soc. Mass Spectrom. 2005;16: 1510–22.
Hsu FF, Turk J. Characterization of phosphatidylinositol, phosphatidylinositol-4-phosphate, and phosphatidyÂlinosiyol-4,5-bisphosphate by by electrospray ionization tandem mass spectrometry: a mechanistic study. J. Am. Soc. Mass Spectrom. 2000;11:986–99.
Hsu FF, Turk J. Structural determination of sphingomyelin by tandem mass spectrometry with electrospray ionization. J. Am. Soc. Mass Spectrom. 2000;11:437–49.
Hsu FF, Turk J. Studies on sulfatides by quadrupole ion-trap mass spectrometry with electrospray ionization: structural characterization and the fragmentation processes that include an unusual internal galactose residue loss and the classical charge-remote fragmentation. Structural determination of sphingomyelin by tandem mass spectrometry with electrospray ionization. J. Am. Soc. Mass Spectrom. 2004;15:536–46.
Hsu FF, Turk J. Charge-driven fragmentaÂtion processes in diacyl glycerophosphatidic acid upon low-energy collisional activation: a mechanistic proposal. J. Am. Soc. Mass Spectrom. 2000;11: 797–803.
Chen S, Subbaiah PV. Phospholipid and fatty acid specificity of endothelial lipase: potential role of the enzyme in the delivery of docosahexaenoic acid (DHA) to tissues. Biochim. Biophys. Acta 2007;1771:1319–28.
Guan Z. Discovering novel brain lipids by liquid chromatography/tandem mass spectrometry. J. Chromatogr. B. 2009;877:2814–21.
Brugger B, Erben G, et al. Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass specteometry. Proc. Natl. Acad. Sci. USA. 1997;94:2339–44.
Kakela R, Somerharju P, Tyynela J. Analysis of phospholipid molecular species in brains from patients with infantile and juvenile neuronal-ceroid lipofuscinosis using liquid chromatography-electrospray ionization mass spectrometry. J. Neurochem. 2003;84:1051–65.
Gao F, Tian X, et al. Analysis of phospholipid species in rat peritoneal surface layer by liquid chromatography/electrospray ionization ion-trap mass spectrometry. Biochim. Biophys. Acta 2006;1761:667–76.
Pang LQ, Liang QL, et al. Simultaneous determination and quantification of seven major phospholipid classes in human blood using normal-phase liquid chromatography coupled with electrospray mass spectrometry and the application in diabetes nephropathy. J. Chromatogr. B. 2008;869:118–25.
Pruzanski W, Lambeau L, et al. Differential hydrolysis of molecular species of lipoprotein phosphatidylcholine by group IIA, V and X secretory phospholipase A2, Biochim. Biophys. Acta 2005;1736:38–50.
Grandois JL, Marchioni E, et al. Investigation of natural phosphatidylcholine sources: separation and identification by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS2) of molecular species. J. Agric. Food Chem. 2009;57:6014–20.
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Chen, S. (2011). Lipidomics of the Nervous System: Phospholipidomics by Liquid Chromatography Coupled to Mass Spectrometry or Tandem Mass Spectrometry. In: Li, K. (eds) Neuroproteomics. Neuromethods, vol 57. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-111-6_19
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DOI: https://doi.org/10.1007/978-1-61779-111-6_19
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