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High-Throughput High-Content Functional Image Analysis of Neuronal Proteins Implicated in Parkinson’s Disease

  • Eva Blaas
  • Ronald E. van KesterenEmail author
Protocol
Part of the Neuromethods book series (NM, volume 57)

Abstract

Parkinson’s disease (PD) is characterized by the progressive loss of dopamine neurons. Here, we describe how to use human SH-SY5Y neuroblastoma cells as an in vitro cell model to study the effects of candidate PD susceptibility genes on dopamine neuron differentiation and viability. This cell model can be used to study the effects of siRNA-induced gene knockdown, either alone or in combination with PD-related cellular stressors such as MPP+, and is compatible with high-throughput cellular screening platforms such as the Cellomics ArrayScan VTI HCS Reader.

Key words

Parkinson’s disease Neurodegeneration Cellular screening High-content analysis SH-SY5Y 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine 

Notes

Acknowledgments

This work is supported by TI Pharma (project T5-207: Parkinson and Alzheimer disease: from dysregulated human brain targets towards novel therapeutics).

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive ResearchVU UniversityAmsterdamThe Netherlands

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