High-Throughput High-Content Functional Image Analysis of Neuronal Proteins Implicated in Parkinson’s Disease
Parkinson’s disease (PD) is characterized by the progressive loss of dopamine neurons. Here, we describe how to use human SH-SY5Y neuroblastoma cells as an in vitro cell model to study the effects of candidate PD susceptibility genes on dopamine neuron differentiation and viability. This cell model can be used to study the effects of siRNA-induced gene knockdown, either alone or in combination with PD-related cellular stressors such as MPP+, and is compatible with high-throughput cellular screening platforms such as the Cellomics ArrayScan VTI HCS Reader.
Key wordsParkinson’s disease Neurodegeneration Cellular screening High-content analysis SH-SY5Y 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
This work is supported by TI Pharma (project T5-207: Parkinson and Alzheimer disease: from dysregulated human brain targets towards novel therapeutics).
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